CRISPR 101: Multiplex Expression of gRNAs
Type
Blog Post
...Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.” Genetics 200(2) (2015): 431-441. PubMed PMID:...Multiple Lentiviral Expression Systems (MuLE) Kit:
This kit can be used to create lentiviral vectors expressing...Assembly. Using BsaI, gRNAs are cloned into one of 12 pYLsGRNA plasmids, which contain various promoters and ...CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014)...