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We narrowed to 82 results for: lentiviral construct

Showing: 41 - 60 of 82 results

  1. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents Addgene...and New England Biolabs sponsored the capital construction of our new BL2+ laboratory and the infrastructure...establish consistent, high-quality protocols for lentiviral production. Bryan Roth’s lab from the University...the Addgene Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral...

  2. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ... CRISPR pooled libraries have been created in lentiviral systems for mammalian screens, which enables ...for screening of cells that are refractory to lentiviral transduction, now enabling forward genetic screens... voltage changes in worms and have made these constructs available at Addgene. These include Arch(D95N...However, some cell types are poorly transduced by lentivirus, and thus screening with available CRISPR libraries...Primary murine T cells cannot be transduced by lentivirus and have been difficult to manipulate genetically...

  3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...Brunello CRISPRko sgRNA library as pooled library or lentiviral prep! Dolcetto: human CRISPRi sgRNA library While...the Dolcetto library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library ...allowing the screen to be conducted with only two constructs (the other containing dCas9-VP16). Like CRISPRi...function. Find the Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench...

  4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew Endy’s and...19 pandemic, the Krogan lab at UCSF generated lentiviral vectors expressing SARS-CoV-2 open reading frames...all but one are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for...(2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited...

  5. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ...plasmid and the baculovirus construct, called a bacmid. Like AAV and lentiviral packaging plasmids, bacmids...-293T cells. If you titrate your viral preps, lentiviral titration requires an additional couple of days...require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus...cells Viruses AAV and lentivirus Baculovirus; can produce AAV and lentivirus if needed # of plasmids...Overcoming the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on...fast-growing, and widely available. The viruses Lentiviruses, AAV, and adenoviruses can all be produced in...cells. However, it is much more common to make lentiviruses and AAV in-house. High-titer adenoviruses are...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A...-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression...same multicistronic vector, effectively making a construct that expresses multiple unique genes at equivalent....) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...generating a series of single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf... expression and labeling approach using these constructs in HEK 293 cells (Smith et al., 2024). They then... license.  The team deposited an array of constructs for tagging the kinesins KIF5A and KIF5B as well...Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether, if you want ...proportions from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). ...

  9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...One good system to balance these needs is the lentiviral-based expression of floxed dCas9 so that it may... for cells stably and highly expressing these constructs can be achieved by fluorescence-activated cell... stable gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin...

  11. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...from three to two. Lentiviral delivery of BioTAP-tagged dCas9 and gRNAs. Using lentiviral delivery of the...  A second application for CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction... BirA, and 3) one or more gRNAs Two separate lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...microtubule severing. A) Schematic of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and ...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...is optogenetically recruited by different CIB constructs to the plasma membrane or lysosomes (the earlier...earlier article also provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4)...100x variants. But those super-high efficiency constructs are great for in vitro biocatalytic applications...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo tools can...gene-of-interest, all the way to the complex multi-TU constructs that compose genetic circuits and metabolic pathways...MoClo is commonly used to design TUs and multi-TU constructs for synthetic biology applications in bacteria...individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016). The variety of constitutive...multiple unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for...is geared towards cloning multi-TU expression constructs (Martella et al., 2017). If you can dream up ...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...

  14. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...efficiency was not affected by CellTagging. By constructing lineage trees and comparing them with the gene...reprogramming and differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101...

  16. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed Addgene...

  17. Adenovirus Plasmids

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    Collection
    ...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...about: Adenoviral components Adenoviral plasmid construction Frequently asked questions about adenovirus ...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

  18. Retrovirus Plasmids

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    Collection
    ...Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...cytoplasm Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct for miRNA expression Chen 52114 pMSCV-IRES-mCherry...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...

  20. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...
Showing: 41 - 60 of 82 results