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We narrowed to 86 results for: lentivirus expression plasmid

Showing: 41 - 60 of 86 results

  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...cell page Find plasmids for reprogramming and differentiation Check out our lentivirus guide Sign up to...), now allows scientists to identify the gene expression patterns unique to the few cells that successfully...Unfortunately scRNA-seq only captures the gene expression of cells at a single time point, making it difficult...difficult to investigate how early expression patterns influence reprogramming outcomes. CellTag from ...single-cell level, thus linking ancestral gene expression patterns with reprogramming outcomes (Biddy et...CellTags were detected over a 10-week period. Their expression decreased over time but they were not silenced...MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and Hnf4α, a process...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-...to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio that gives...drives expression of puromycin. Puro R Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian...shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral... also be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg...into the pLKO.1 vector, producing a final plasmid that expresses the shRNA of interest. C.1 Recommended ... transient knockdown of protein expression, you may transfect plasmid DNA directly into the target cells...

  3. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...and lentiviral expression vectors (Table 1). The four systems Name Function Plasmid Transduction...deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first ...2019b). CHIME works through transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow in...

  4. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...factors (TFs) act as the gate keepers for gene expression, turning transcription on and off by binding ...binding proximal to their target genes. Since gene expression patterns determine everything from stem cell differentiation...(aka the MORF Collection) and an accompanying expression atlas, including over 3,500 human TF isoforms...TF library, with each cell receiving only 1 TF expression construct. Cells were cultured following a differentiation...from Joung et al.)    This atlas linked TF expression to differentiation outcomes as well as global...reference cell types generated by the human fetal expression atlas. Computational analysis was used to infer...

  5. 27 Hot Plasmids from 2016

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    Blog Post
    ...transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila and yeast...Cas9 nickase, and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. Ordon et al., 2016 tested...Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ... you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over...over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    ... have found that expression occurs quickly and results in long and stable expression (up to 9 months in...the onset of gene expression. However, it has yet to be determined what gene expression looks like at 3 ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...AAV2 can transduce many tissue types, it has low expression in several brain regions where other serotypes... under study. Many AAVs will show significant expression in a variety of cell types, but you should try...transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport is fine and...

  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...Download our Plasmids 101 eBook to get all the background you need to start working with plasmids! What are...terminators are not usually employed in plasmid-based expression systems, so these will not be detailed...at the end.  Nearly all common bacterial expression plasmids use Rho-independent terminators, which include...termination with polyadenlyation. Mammalian expression plasmids are primarily used to create mRNA and the...Polyadenylation Signal Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts Resources...

  8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...donor plasmid or single-stranded DNA oligonucleotide, so you will need to make sure your expression system...included with each expression system as well as suitable applications. Expression System Components ...System Application Mammalian expression vector Promoter driving Cas9 expression can be constitutive or inducible...or electroporation. Transient expression of CRISPR components, expression decreases as RNA is degraded ...using cationic lipids. Transient expression of CRISPR components, expression decreases as gRNA and Cas9 protein... provide an overview of some CRISPR mammalian expression systems, the typical applications for each, and...gene? Do you want to activate or repress gene expression? Are you trying to create a single point mutation...

  9. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...promoter, which is used to drive the expression of the array. After expression, the individual gRNAs are released...demonstrated that the tRNA-gRNA array can drive the expression of itself, without using any additional promoter...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ..., that rely on an upstream lengthy promoter to express the gRNA array, DAP arrays use tRNA itself, as ...tRNA-gRNA arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716).   ...resources on the Addgene blog CRISPR 101: Multiplex expression of gRNAs Four Base Editing Reporters to Monitor...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...applications Gene expression control Researchers are always wanting to manipulate gene expression – turn it up...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral... the genome of the target cell or transiently expressed. It’s important to note that for all the applications...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...Viral expression cassettes typically contain a promoter (inducible or constitutive), your gene of interest...and often a selectable marker. This vector can express your construct in target cells transiently or constitutively...

  11. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ... smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system...technology.” GeCKO library for genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also... thousands of targets at once. That’s what our plasmids allow.” Wang’s library is designed to target a...have previously been stably transfected for Cas9 expression. The Yusa lab has used their library to screen...

  12. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...provide synthesized SARS-CoV-2 expression plasmids available for many expression systems: bacterial, yeast,...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate... pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids used for this ...spike protein to improve expression and pseudotyping efficiency. This plasmid was later used by another...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from...

  13. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... to co-express them from one promoter. Specificity The ability of AAV to drive gene expression in specific...cell-specific gene expression and viral infection. Cre or Flp dependent switches control gene expression via switches...transgene expression, since you don’t have to wait for the host to replicate the DNA before expression. Now...non-enveloped icosahedral capsid, that can be used to express genes of interest in cell and animal models. AAVs...laboratory experiments ranging from transient gene expression in a particular cell type to CRISPR genome editing...genes, you can coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates.... On the other hand, if you’re interested in expressing multiple small genes, you can design single rAAV...

  14. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...transgene expression. Once packaged in AAV, this pre-recombined DNA could lead to transgene expression in recombinase-negative...sufficient transgene expression but limits the likelihood of recombinase-independent expression. Next-generation...Ready-to-Use AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ...serotype. Additionally, we check viral transgene expression in transduced cells whenever possible. This post... tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during the... human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside the ...) are aligned to the reference sequence of the plasmid used to create the viral prep. In a clean viral...

  15. Viral Vectors 101: Biosensors

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    Blog Post
    ...analyte), and accumulation of ATP activated GFP expression (receptor), then the transducer could be a microscope...genetically encoded, and luckily, we have pre-designed plasmids for these systems! Ion sensors One common biosensor...retrovirus, can either permanently integrate and/or express long term (up to several years!). Biosensors tend...biosensor to specific tissues or cell (sub)types for expression and downstream monitoring. Nervous about making...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...we’ve got you covered with packaging plasmids! References and Resources References  Bhalla N., Jolly ... as stable systems, rather than transiently overexpressing at the time of use. Another great viral perk...

  16. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...precisely control where and when certain genes are expressed to investigate their role in a specific behavior...Which viral vector should be used? Modifying gene expression Generally speaking, one may want to achieve either...application.  Genetic manipulation can also reduce expression levels of certain genes. A popular method uses...allows scientists to draw conclusions due to gene expression in a specific brain area, avoiding side effects...targeting the gene is cloned into a viral shRNA expression vector and the vector particles are delivered...delivered into the brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for... a tight spatial and temporal control of gene expression manipulation. They are injected using a stereotactic...

  17. Plasmid Collections

    Type
    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 162,409 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins...

  18. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses the activation helper protein. SAM can ...Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind...that will enable even more fine-tuning of gene expression levels and on software for analyzing the data...you are equipped and authorized to make and use lentivirus. Also, note that due to the size of the backbone...

  19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors. This...after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that...

  20. Hot Plasmids - November 2023

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. .... Today, it's time for another edition of Hot Plasmids!  Here's what you'll find in this post:  New...., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023).... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D., et al. (2023). Cell type-specific...pulse-chase experiments, and more. Find LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID...LOV-Turbo into TransitID experiments.  Find TransitID plasmids here! These two developments open up new possibilities...
Showing: 41 - 60 of 86 results