We narrowed to 121 results for: mouse c rel

...-018-0219-4  Yu C-C (Jay), Barry NC, Wassie AT, et al (2020) Expansion microscopy of C. elegans. Elife...easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans (Damstra, et...physically separates molecules while maintaining their relative positions, allowing you to resolve individual ...accessible imaging equipment, ExM protocols utilize relatively inexpensive reagents and are readily adoptable.../doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical guide to ...they want to understand the ultrastructural relationships that influence everything from cell division...optimization and careful execution, but they cannot be entirely eliminated. It is recommended that you image samples...

...difficult to grow. Growth Temp Choose from 30 °C, 37 °C, or room temperature. Growth Strain Please... Choose from: H. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus (chicken), B. taurus...expression, insect expression, plant expression, mouse targeting, CRISPR, TALEN, synthetic biology, affinity...frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast)...be indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid change as ...

...these attributes of Cre-lox to create the Brainbow mouse neural labeling system. Brainbow-1 and -2 have been...Brainbow constructs into mice. When they imaged the mouse brains, they found many more than 3-4 colors due... of limitations. First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity...  Brainbow variants and applications beyond the mouse brain In addition to improving the Brainbow system...Sato T, van Es JH, van den Born M, Kroon-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers...melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. ...observed in a given area. In 2013, Dawen Cai, et al. released a refinement of the Brainbow technology, Brainbow...

...Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, or C base editors...transversion editors: CGBEs, or C → G Base Editors. CGBEs first convert a cytosine (C) to uracil (U), just like... a more specific A → C base editor (ACBE), they inserted the deaminase into the C-terminal domain of Cas9...pyrimidines (C ↔ T).   Base transversion Base change from purine (A or G) to pyrimidine (C or T) or vice-versa...type of base editor invented. CGBE C → G Base Editor Converts C → G by fusing a uracil DNA glycosylase..., or C, respectively, to be replaced with another base. References Chen, L., Hong, M., Luan, C., Gao... Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C., Zhu, Y., Guan, Y., Wang,...

...deposit please sign up here.        A retroviral mouse CRISPR knockout library Article contributed by Alycia... factors as the screen readout. This retroviral mouse CRISPR pooled library can be used for screening ...screens in cells with few genetic tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et...this may be due to different lipid compositions of C. elegans membranes, which might beneficially influence... of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect measurement...that the system is active in E. coli and closely related species, is amenable to use with other bEBP/UAS...and the system may not be adaptable to distantly related species from E. coli. This tool should serve as...

...12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor...in the primary somatosensory cortex (S1) of the mouse brain following retro-orbital AAV injection at 4...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin...integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim, J., Hiraizumi, M., … Abudayyeh... Cenik, B. K., Aoi, Y., Iwanaszko, M., Howard, B. C., Morgan, M. A., Andersen, G. D., Bartom, E. T., &.../doi.org/10.1016/j.molcel.2024.11.007. Branon, T. C., Bosch, J. A., Sanchez, A. D., Udeshi, N. D., Svinkina...

...differences in chemogenetics and optogenetics in a mouse experiment. Viral delivery is the same in both cases...light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent intracranial...annurev-pharmtox-010814-124803 Vlasov, K., Van Dort, C. J., & Solt, K. (2018). Optogenetics and Chemogenetics...level of control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs...throughout the body. Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation... of being less invasive and more flexible as it relies on designer ligands that can be simply introduced...

...well? Our collection includes a mouse and a chimeric rabbit (original mouse Fab region with rabbit Fc) anti-FLAG... al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted genome editing...enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered to brain endothelial cells in...mChe), with co-incubation with assist peptide (AP). C) Workflow for Cas-RNP-PAGE editing of primary T cells... and minimal toxicity, including in clinically-relevant lines such as CAR-T cells. Plus, this method is...

...stronger fluorescent signals upon sensing glutamate in mouse and ferret models. Furthermore, they created three...showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation ...Improved trafficking and localization through different C-terminal anchoring domains The original iGluSnFR in...adapted from Aggarwal et al… Figure 4. PDGFR is a C-terminal fusion to the PDGFR transmembrane domain ...mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains...domain, followed by a modified form of the cytosolic C-terminal domain of Stargazin including a terminal ...give researchers the ability to monitor glutamate release from neurons and other brain cells in vivo. Glutamate...

...indicator which had improved performance in live mouse brains. Currently, she is using the improved voltage... indicator coming from the screen to understand mouse cortical and hippocampal neural microcircuits. “...: Citrine-tagged voltage indicator expressed in mouse cortex. Image from He Tian. Congrats again ...stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses in the left and right...Michael Davidson and Roger Tsien who have played enormous roles in the development and dissemination of ...This biosensor changes fluorescence based on the release of glutamate from neurons and this new version ...iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at once. “This work has...

...recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable to other...labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling of the...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal ...Feng Q, Zhou Y, Zeng S, Ren M, Jiang S, Ni H, Zhou C, Gong H, Luo M (2018) Cell-type-specific and projection-specific...these labor-intensive and invasive techniques to reliably label any post-mitotic cells in Drosophila. SPARC... be labeled in a given population is directly correlated to the length of the truncated attP site. The...

... one gene and activate another. In the field of mouse genetics, FLEx is currently being used to study ...really due to a single gene deleted in your knockout mouse, you can design a FLEx switch conditional knock-...genOway press release for more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling...Cre-mediated recombination at the cellular level in the mouse. 2003 PubMed PMID: 12665802. 2. Branda, CS and Dymecki...

...Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov, R., Fort, A., Gebhard, C., Goldowitz, D., Guhl,...Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Potent and specific genetic interference...raw %}{"no_follow":false,"open_in_new_tab":true,"rel":"noopener","sponsored":false,"url":{"content_id"...atlas of miRNAs and their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org...10.1038/sj.emboj.7600385 McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning strategies for constructing...6750-6-1 O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms....1080/15476286.2023.2288741 Taxman, D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short ...

...ideal for whole animal administration, such as in mouse experiments. AAV also has multiple serotypes (12...induce a lower inflammatory response within hosts. Relative to the other viruses discussed in this article...10.1016/j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...2011 Jun;3(6):677-713. DOI: 10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of...lentivirus and gamma-retrovirus are so closely related, their required components are very similar. While...

...transmission in brain slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split... present, it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to...chain of BoNT/B into two fragments (BotNT-N and BotNT-C) and joined each fragment with one part of an established... it inactive, thus inhibiting neurotransmitter release. How is this light-mediated? The researchers split...visualizing the location and function of microbes rely heavily on fluorescent protein genes. These optical...

...anti-mouse CD11b-BV510), which is present on dendritic cells but not T cells, and CD8 (anti-mouse CD8b-PerCP-Cy5.5...experiment. Practical example: lymphocytes in a mouse tumor sample You made it! So much about the theoretical...where I harvested a melanoma tumor from a C57BL/6 mouse and analyzed the tumor-infiltrating lymphocytes ...Using a practical example of lymphocytes from a mouse tumor, you’ve learned how to read an FSC/SSC plot...in fewer cells being considered for the analysis. (C) A more liberal gate allows more cells to be considered...looser gate here. I am not worried about including irrelevant events in my analysis, as these will get excluded...from your single cells, as duplicates can’t be reliably analyzed on their staining signals. For this, ...

...another popular model organism. A close relative to the mouse, both are great mammalian models due to .../000504294 Wang, T., Guan, C., Guo, J., Liu, B., Wu, Y., Xie, Z., Zhang, C., & Xing, X.-H. (2018). Pooled...that make them an ideal model organism; they are relatively simple to maintain and breed and they have large...doi.org/10.13918/j.issn.2095-8137.2016.4.237 McCusker, C., & Gardiner, D. M. (2011). The Axolotl Model for ...elife.50651 Vieira, W. A., Wells, K. M., & McCusker, C. D. (2019). Advancements to the Axolotl Model for ...BSL-1 status, E. coli can be used as a model to relate these processes in more pathogenic bacteria (Blount...regeneration.  In addition, axolotls have many age-related phenotypic changes that are similar to those observed...

...organisms and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations...Importantly, since Protein A has weaker affinity for mouse IgG, it is helpful to incubate with a rabbit secondary...digestion, fragments are released from nuclei by a short incubation at 37 °C. These short DNA fragments...or more transient DNA-protein interactions, the reliance on fixation does increase the chance of false-..., which stands for ‘Cleavage Under Targets and Release Using Nuclease,’ was recently developed by the ...strong detergent to permeabilize cells rather than to rely on nuclei extraction. They report that this change...

...throughout the brain. Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive....2019.02.037 Kagiampaki, Z., Rohner, V., Kiss, C., Curreli, S., Dieter, A., Wilhelm, M., Harada, M., Duss...References and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H.,...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors...

...these tools because obviously, unlike the mouse, we can’t rely on genetic engineering to optimize humans...to fuse the activators instead of at the protein’s C terminus. With that new design in place, Konermann...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki...Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann ... not managed to create a system to allow us to reliably activate essentially any gene,” she says. The ...