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We narrowed to 449 results for: organization Addgene

Showing: 41 - 60 of 449 results

  1. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...different organisms. There are several codons that encode the same amino acid. Each organism, however,...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...the genetic code is universal, many different organisms actually prefer certain codons over others for... biologist who wants to express genes from one organism in another? Let’s take a look at codon usage and...optimize codons for expression in a particular organism. Why is codon usage important? Translation and...for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization...expression by changing synonymous codons based on an organism's codon bias. The idea is that scientists can make...

  2. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends... tools also requiring that the user specify an organism. Output diversity: Different tools provide results...large genome”, and “organism biased scoring”. Trends: Many tools can handle genomes of any size and are...

  3. Viral Vectors 101: Virus Safety

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    Blog Post
    ...Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide Addgene Viral Plasmids ...from organization to organization, so you’ll need to be familiar with your specific organization/department...-2 labs. When working with lentivirus, many organizations require or recommend disposable gowns over your...cabinet, even if it is not required by their organization. It’s always okay to add another level of protection..., 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage of...cut. Following all sharps procedures for your organization will significantly reduce the risk of an accident...also need to familiarize yourself with your organization’s reporting mechanisms before you start an experiment...

  4. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post
    ...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst...great organization to develop these leadership skills in particular because every year it organized a “Committee...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ... achieved by volunteering for a professional organization or a scientific society in your field. My leadership... in the long run was most beneficial for the organization as a whole. I came to understand that not only...only was I growing as a leader but the organization was growing too and with that would come necessary... had to be professional as I represented the organization, not just myself as a scientist. I learnt to...

  5. Engaging with science and society at pgEd

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    Blog Post
    ... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...different organizations and individuals, to identify ways of collaborating, and to organize events that...professional development (PD) workshops for teachers; organizing Congressional briefings in Washington DC; convening...Hollywood; and working directly with community organizations, libraries, youth groups, and faith institutions...studies (STS). I also worked with a number of organizations in public engagement efforts, including giving...middle and high school students. One of these organizations was pgEd. After obtaining my PhD and working...audiences. Many universities or cities have organizations (often volunteer-based) that put on public lectures...

  6. Introducing Addgene's Microbiology Pages

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    Blog Post
    ...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...us know about other organisms we should include by emailing us at help@addgene.org. If you are looking...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology...plasmids used in E. coli and yeast, you’re in luck! Addgene has so many of these resources that we created ...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...technology, sign up to write about it on the Addgene blog. ...

  7. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ...Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic Biology Collection...Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy... a variety of functions that allow their host organisms to thrive in competitive environments. The genes...the competition, and Penicillium isn’t the only organism that knows it. Some bacteria possess plasmid-borne...encode genes that allow the host to infect other organisms. If you’ve ever wondered why E. coli is safe to...means to deliver DNA and other molecules to host organisms. For instance, vectors derived from the Agrobacterium...like by living where no one else wants to. Some organisms manage this with plasmids that allow them to eat...

  8. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

  9. K. phaffii: Rising to the Occasion

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    Blog Post
    ...path.   Yeast as a model organism Yeast are single cell, eukaryotic organisms formally classified as fungi... Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ...know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable...cerevisiae (budding). Yeast make for an excellent model organism for a few reasons: Affordable to culture and... methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model for peroxisome...et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-...studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain enzymes and...

  10. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    Blog Post
    ...nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like...pantry or community arts organization. Many people are surprised to learn that Addgene is officially filed,... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...partnerships with organizations that share our community-oriented values. Addgenies like the nonprofit...

  11. How Do I Place an Addgene Order Using a PO Number?

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    Blog Post
    ...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the...Anyone affiliated with the recipient organization can create an Addgene account and place an order: a purchasing...through your organization in this field. Please also enter the billing address for your organization. We will...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...plasmids online is a little trickier -- but at Addgene, we try to make the process as smooth and simple... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that...

  12. Why Do I Need an MTA Anyway?

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    Blog Post
    ... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...

  13. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post
    ...reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global...

  14. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

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    Blog Post
    ... first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene board member. When...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of Directors...We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief...experience and insight to the now 13 member board at Addgene. Let’s meet the newest board member and get to ...School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join...serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great time for...

  15. Science Communication Snapshot: DayCon 2015

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    Blog Post
    ...as a free event! Organizing a science conference for the public How does one organize a public scientific... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate... graduate student organization Science in the News (SITN), we recently sponsored a first-time event called...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...terms of cost and content level. Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made... do you share your science with the community? Addgene would love to hear about your efforts to engage...opportunities to improve our communication and organizational skills provide great training for a number ...

  16. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post
    ...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...          Click Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration...

  17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post
    ...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...

  18. Our Most Popular Blog Posts from 2019

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    Blog Post
    ...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm....

  19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post
    ...body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak peak...fluorescent microscopy - even in living cells and whole organisms. You can take advantage of the many Fluorescent...pathways, it is possible to map the internal organization of a cell. This mapping can be done by using antibodies...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ... proteins” which are known to be a part of an organelle or involved in intracellular transport pathways...for commonly used markers for major mammalian organelles and transport pathways. It is best, though not...

  20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post
    ...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of blog... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation... additional resources and information about organizations promoting reproducibility in this booklet (which...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...within a published manuscript. Luckily, many organizations have been working to provide tools that make...
Showing: 41 - 60 of 449 results