We narrowed to 466 results for: organization Addgene
- 
  TypeBlog Post...path. Yeast as a model organism Yeast are single cell, eukaryotic organisms formally classified as fungi... Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ...know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable...cerevisiae (budding). Yeast make for an excellent model organism for a few reasons: Affordable to culture and... methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model for peroxisome...et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-...studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain enzymes and...
 - 
  
Institute for Protein Innovation
TypeCollection...secreted proteins from a collaboration between Addgene and IPI....animal immunization. IPI is a nonprofit research organization with a mission to advance protein science to...Other Markers Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope... - 
  
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like...pantry or community arts organization. Many people are surprised to learn that Addgene is officially filed,... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...partnerships with organizations that share our community-oriented values. Addgenies like the nonprofit... - 
  
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the...Anyone affiliated with the recipient organization can create an Addgene account and place an order: a purchasing...through your organization in this field. Please also enter the billing address for your organization. We will...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...plasmids online is a little trickier -- but at Addgene, we try to make the process as smooth and simple... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that... - 
  
Why Do I Need an MTA Anyway?
TypeBlog Post... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ... - 
  
CRISPR Plasmids for Genomic Visualization
TypeCollection... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... - 
  
Structural Genomics Consortium Plasmids
TypeCollection...Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional...Oxford and Stockholm sites have been deposited at Addgene and are available to request. Browse the SGC Plasmid... - 
  
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global... - 
  
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post... first hear about Addgene? I heard about Addgene from Joanna Brownstein, an Addgene board member. When...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of Directors...We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief...experience and insight to the now 13 member board at Addgene. Let’s meet the newest board member and get to ...School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join...serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great time for... - 
  
Cre-Lox and Other Site-Specific Recombinases
TypeCollection... the expression of specific genes in cells or organisms. Each recombinase enzyme recognizes and binds ...sequence of the recombinase to align with your organism's preferred codon usage will improve expression...FLEx switch and what you can do with it, read Addgene's blog on FLEx Vectors . Flp-IN: A single FRT site...Description Vector Type Promoter PI Additional Addgene resources Explore our full catalog for plasmids... Packaged on Request service for more options. Addgene blog Plasmids 101: Cre-lox Cre-ating New Methods...Description Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids ... Packaged on Request service for more options. Addgene blog Advanced Uses of Cre-lox and Flp-FRT — A Neuroscientist... - 
  
Science Communication Snapshot: DayCon 2015
TypeBlog Post...as a free event! Organizing a science conference for the public How does one organize a public scientific... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate... graduate student organization Science in the News (SITN), we recently sponsored a first-time event called...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...terms of cost and content level. Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made... do you share your science with the community? Addgene would love to hear about your efforts to engage...opportunities to improve our communication and organizational skills provide great training for a number ... - 
  
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for... Click Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration... - 
  
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based... - 
  
Our Most Popular Blog Posts from 2019
TypeBlog Post...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm.... - 
  
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... - 
  
CRISPR Plasmids - Cascade-Cas3
TypeCollection... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... - 
  
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... - 
  
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak peak...fluorescent microscopy - even in living cells and whole organisms. You can take advantage of the many Fluorescent...pathways, it is possible to map the internal organization of a cell. This mapping can be done by using antibodies...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ... proteins” which are known to be a part of an organelle or involved in intracellular transport pathways...for commonly used markers for major mammalian organelles and transport pathways. It is best, though not... - 
  
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of blog... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation... additional resources and information about organizations promoting reproducibility in this booklet (which...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...within a published manuscript. Luckily, many organizations have been working to provide tools that make... - 
  
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post... development. These are required for a growing organism to make the complicated transition from a clump...dynamic processes are largely absent in adult organisms, where tissues structures are more stable and ...movement from the bone marrow and other lymphoid organs to specific tissues where they can scan for any...is large scale cellular movement throughout an organism. Studying cell mobility within these complex...tools to directly follow immune cells in lymphoid organs. The development of cell sorters together with ...antibodies made it possible to track cells from one organ to another. Thus several labs were able to decipher...microscopy images of chemically coloured lymphoid organs did in the past. How do researchers track fluorescently...