We narrowed to 166 results for: pcr

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Learn what a high fidelity DNA ligase is and when you might need one.

How much time does it take to clone a plasmid and how much does it cost? We do some back of the envelope math to try and figure it out!

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

Learn how to use recombinase-based state machines from the Lu lab to monitor cellular events and regulate gene expression.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

Ready to produce rAAV in your own lab? This 101 post can help!

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

Learn more about Addgene's NGS verification process for pooled libraries using real-life amplication data from the Brunello gRNA library.

Addgene's new cloning grade DNA service allows you to start cloning right when you receive the plasmids from Addgene. No amplification and extractions necessary!

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Find out what were Addgene's most popular blog posts in 2020!

Learn how you can use planarian plasmids from the Sánchez lab to teach developmental biology techniques to new molecular biologists.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

CRISPR plasmids for endogenous tagging of your gene of interest.