We narrowed to 208 results for: pcr

...protocol is surprisingly simple, requiring only a PCR product containing the modifying sequence flanked...to the chromosomal site of insertion. The linear PCR product is introduced into the cell by direct transformation...selection marker (KanMX, HphMX, or His3MX).  Each PCR product from this collection will yield an insert...

...sample. The PCR is then performed, optimized as needed, and cleaned up using a standard PCR cleanup kit...scientists work with the depositing lab to develop a PCR strategy suitable for the library in question. We...that can amplify the insert of the library. This PCR step also adds the necessary adapter sequences for...intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries. A broader...

...primers to create 200-300 bp sequencing products using PCR. A diagram of the product we created for the Brunello...library is shown in Figure 2. You can use a small PCR product because the plasmids in the library are identical...both pre- and post-amplification DNA and generates PCR sequencing products from both. These products are...

...1999), quantitative PCR (qPCR) (Aurnhammer et al., 2012), droplet digital PCR (ddPCR) (Lock et al., 2014...Ehrhardt, A., & Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated...adeno-associated viral vector genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https... experimental outcome and planning.  Both ddPCR- and qPCR-based titering assays measure physical titers...containing a viral genome. While ddPCR is superior in precision and variability, qPCR is the more widely used ...more details on the qPCR titering process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re...interested in the ddPCR titering process, which is what Addgene uses, check out our ddPCR for AAV quantitation...

...from Addgene you could directly start your digest, PCR, or transformation?  Great news. A subset of our ...biology experiments such as cloning, digestions, PCR etc. and/or directly long term storage. Every preparation...

...7 days. The crRNA and tracrRNA are annealed on a PCR block and the annealed cr:tracrRNA is then mixed ...using the csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow when...Assemble csRNP: Anneal crRNA and tracrRNA using a PCR block. Add Cas9 protein to the annealed cr:tracrRNA...well plates: Characterize clonal lines by ICC and PCR genotyping (Sanger sequencing); use primers flanking... the tag sequence and expect a size-shift in the PCR product for knock-in lines. Troubleshooting low ...alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely failed. If ...

...codon. You can domesticate the sites yourself using PCR, but gene synthesis can save you time. Using gene...transforming into chemically competent cells in a 96-well PCR plate. Always confirm your results Congratulations... 8–10 colonies. You can check colonies either by PCR, by directly extracting and sequencing the plasmid...and direction (which is not easy to see in colony PCR electrophoresis), is free of undesired mutations,...

...report that one can use linear repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms ...In the background are 504 restriction digests of PCR products used to identify a single 2xFLAG knock-in...used to generate linear dsDNA repair templates by PCR boosted efficiency (Ghanta et al., 2018). They further...screening for knock-ins much more efficient. Pure PCR repair templates are critical for efficient knock-ins...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured...

...in the original plasmid (Option 1) or added using PCR-based amplification (Option 2). Advantages of Golden...work around this is to "domesticate" your fragment: PCR-based amplification can be used to create silent ...thus eliminating these from your gene of interest. PCR products are then digested with the Type IIS enzyme...

...Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch to droplet digital PCR to quantify...

... AAV particles are titered by droplet digital PCR (ddPCR) using primers and probes targeting the ITR elements...April 2016) also performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures high quality...are titered using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified from Wang et al...confirmed to have the correct integrated provirus using PCR. This assay involves transducing cells with serial...obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering...

...create dsRNA. Students can produce DNA templates via PCR off of the same plasmid and use these to create riboprobes...can easily generate off of a circular plasmid via PCR. Typically, to make these constructs, developmental... create a complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the...

...used for mycoplasma detection is using PCR. In this technique, PCR primers specific to the 16s RNA genes... you know it’s there? For this task, you can use PCR based detection, DNA staining, fluorescent tagging...Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. J Microbiol...

...TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq...

...homology required by MMEJ can easily be added via PCR amplification. Given the inefficiency of HDR for ...microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the PITCh vector... 4: Select for puromycin resistant cells Step 5: PCR amplify and sequence the locus to verify correct ...

...inversion creates a template that’s suitable for PCR amplification using pre-designed primers. By using...interest. Repeat rounds of infection and screening by PCR enrich for capsid variants with increased ability...those that cross the barrier can be identified by PCR later. The PNS presents a different problem: it’s...

...oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification of specific DNA sequences...

...pairs.  dsDNA Substrate dsDNA inserts can be made by PCR using primers that amplify the DNA sequence of interest...recombineering substrates to avoid errors introduced during PCR. ssDNA Substrate ssDNA substrates can be generated...ordering synthetic oligonucleotides or denaturing short PCR products. Either way, the substrate should be ~70...the presence of the desired modification. Colony PCR can be used to screen for positive clones in most...

...for PCR and surveyor. Q15: I used DNA polymerase Takara Ex Taq™ DNA Polymerase for my genomic PCR, but...conditions with 5% DMSO or without 5% DMSO. My genomic PCR didn’t work, do you have any suggestions? A14: The... protocol, then uses around 2–4 µl in each 50 µl PCR reaction. The gDNA extracted in this way is usually...groups have successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this...

...definition) in a specific field.  For example, while “PCR test” has recently transitioned from jargon to non-jargon...jargon in most other uses. Many laypeople would use “PCR test” to mean “a test that uses a biological sample...