...seriously. As I make my rounds speaking at research institutions and at scientific conferences, I see that ...coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't tell you...it.  Because I am located in Cambridge, MA (and because we have a lot of scientists here) I know many coaches...speaking engagements as are many of the coaches I have mentioned. I have seen a lot of fantastic online content...will make you take the experience more seriously and I have seen, without exception, that this helps people...reaching their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer...will cost a fortune for a successful outcome. How do I find a coach? Start with referrals. Are there scientists...

..., or so I thought. So I entered a molecular and cellular biology PhD program in 2011, which I left four...I didn’t plan to get a master’s degree. I wanted to be a scientist and you need a PhD to be a scientist...exactly the type of scientist I wanted to be. But prior to graduate school, I wasn’t sure a master’s was ...more autonomy?  Before my master’s, I worked in an academic lab where I ran a handful of assays over and ...and over. Everyday was the same and I was bored. I wanted more variety and more power to influence the direction...still decide to earn a PhD, but if I had to do grad school over, I would just do the master’s! If you'...degree felt like a consolation prize, but in time I realized its value. My master’s strengthened my critical...

...Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial...SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9...

...unpleasant experience I thought: I could do this job so much better. Driven by that, I successfully applied...junior postdoc I was aware that such roles existed, but did not consider them for myself. I started picturing...biotech companies), and as far as I remember, it was the first time that I was absolutely convinced it was...company’s fault. I contacted them to express my concerns and request a replacement. I got my replacement...picturing myself in a technical support role only when I was confronted one day with a product issue in the...resolved - but it was a rather painful to get there: I had to send the company detailed documentation of ...of my results, answer loads of questions, and I also had to do some troubleshooting as advised by the technical...

...health. As a teenager in high school, I knew that this is what I wanted to do,” Stegmeier says He began...treatment of rare and ultra-rare diseases. “Today, I am a scientist that helps with different aspects of...Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th grade and then... in for a long time. “At an early age, I was fascinated by the impact that medicines can have on human...foray into genetics during graduate school at MIT. “I read some of the seminal papers on cell cycle discovery...the University of Leuven in Belgium. “That’s where I not only got incredibly compelled by wet bench molecular...disrupt these inefficiencies is pretty remarkable. I was compelled to be part of that.” He’s deposited ...

...listening to me. When I got home, I saw my daughters playing with some cards and I had a quick flashback...engagement. I figured, an engaging card game could help me get the engagement I wanted. I therefore started...some other professors, I went home thinking about how to solve this problem. I didn’t want to face another...diseases. That’s when I thought, if it cannot be hands-on, it will be “brains-on”! I know that even lectures...as much as I try being compelling, interactive, and application-driven to boost curiosity, I have never...courses about gene therapy and genetic manipulation, I and other professors at the University of Barcelona...you might have to drastically alter your strategy. I realized that similar types of thinking go into developing...

...Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme nearly three years ago I decided it was time to get...kids, I had put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took...After being with the company for about six months, I learned there was a Genzyme-based running team called...correct treatments, if available. As part of the team, I started adding mile after mile on my poor, poor legs...partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ Gonzales... bone that only further impairs the patient. When I first met AJ and his family, he did not seem much ...supporting AJ, who is a big superhero fan. This year, I have decided to run as The Green Lantern’s friend,...

...USA). I had never participated in a protest before, so I had no idea what to expect. Admittedly, I was ...Below I present perspectives on the March for Science from researchers all over North America. I hope ...funding.  So when I saw that there was going to be a march in support of science, I loved the idea and...38.9072°N 77.0369°W – Mark Lucera I am a passionate scientist.  I care about my work because it's important...86.7816°W- Jennifer Stancill I am a research biologist by training, so of course I felt compelled to participate... hostile anti-science activists. However, I must say that I was supremely proud of Nashville that morning...37.9101°N 122.0652°W – Steph Hays When I marched for science in May, I marched to advocate for the process...

...extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really struggled to get those...information and I believe that a lot of European students and researchers are craving, like I was, for a better...share their plasmids. Now I am on the other side of the force and as an Addgenie I will do all my best to...to help them in their tasks. But I won’t be alone. I join another Addgenie in our office in London, Emma...PhD in Developmental Physiology at the University of Paris VII. During my years in research, I developed...service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach Scientist - the...supporting European scientists. Born a french frog, I moved to Albion six years ago to work as a research...

...of which I could identify. There were dozens of different mushroom varieties alone. At least I could tell...mushrooms. In some cases I couldn’t tell if the bags contained flora or fauna. I tried to find out what ...Having never been to China myself, I was pleased to be able to participate when the organizers of the...to hear current findings in nucleic acid science. I was delighted to see that the majority of attendees...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of ...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran ... Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These single stranded,...

...getting tighter and competition is fierce. However, I believe there are plenty of great jobs out there for...conference is organized by postdocs for postdocs. I am delighted to see enterprising early career scientists...working together on the event planning and execution. I am seeing many similar examples of science trainees...Penn Biotech Group and PBG Healthcare Consulting. I know of many schools that have completely student/...career preparedness training for their scientists. I have worked with some great postdoc and grad student...own hands, there are some easy ways to get started. I am happy to work with any group of trainees interested... creating their own opportunities for career development and networking. Aside from the importance of ...

... of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought there...focus further development toward. Quality control is, however, not a focus of this blog post. I separate sgRNA...CRISPR software and the piñata effect Two years ago I was a part of a group (Biology of Host-parasite Interactions...Plasmodium falciparum (the parasite that causes malaria). I attribute their success to technical expertise, thoughtful...software to use. With so much choice the question I have is, how did we get here in the first place? How...time to assess public response; and, it is the stage I think we are currently at in the CRISPR software space...to succeed. With respect to de novo sgRNA design, I again separate it into two categories. On the one ...

...meeting.  I experienced this at the ASM General Meeting (now Microbe) in 2013 in Denver, CO. While I had been...and colleagues had moved on as well, so I knew very few people. I didn’t really fit in anywhere and everyone... hashtag I had, however, started a Twitter account, and was following the meeting hashtag. I noticed Holly...Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside the room...with me talking about the tools I could use, and some experimental hurdles I would encounter. This was invaluable...folks at the meeting. I didn’t know anything about these people at the time, but I met some very accomplished...job, I was willing to introduce myself to someone new and ask for help. That was NOT something I would...

...listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom. I realized I could and should be interacting...school’s equivalent of a senior thesis), I knew early on that I wanted to work with a partner. WPI’s emphasis...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d spent...mutants by myself, I would have slowly gone crazy. As it was, my partner Mike and I split the work, shared... and I am known by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily...happily embrace (and admittedly, I may have started that nickname myself). I have my own little Product Management...few in my 20+ year career that I can’t help but feel a bit dubious when I do come across one. Also, remember...

...my academic jobs, I interviewed with just the PI of the lab and for one position, I just had a phone interview...lasted for 30 minute to an hour. Although I never experienced this, I heard from friends that Scientist positions...story demonstrates I possess.   Bullet points on resumes are like cliff notes, so I use these as a starting...this information. I found it useful to write my list of questions in a notebook that I took with me to ...familiar to you. It was my experience not long ago. I just recently started working in the pharmaceuticals... four more years in academia. During my job hunt, I quickly noticed one of the biggest differences between... interview. I also transitioned between different lab groups without having a formal interview process...

...Europe, something that I would not able to do without a program like this. However, I realized that this ...program was much more than that. I discovered that the challenges that I am facing in my career are common...Oviedo): “I couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: ...will arrive to Spain at one point, since I am not sure that I want to pursue the academic track.” How ...2014-15, University of Valencia): “At the beginning, I registered at IMP to have an opportunity to improve...with mentors who are at a professional level where I would like to be in a few years, helping me to advance...boundaries for both professional and personal development, is essential to excelling in the modern world...

...facilitate your company’s development  Don’t be afraid to email strangers asking to meet. I have cold emailed ...background and/or connections to even begin this process. I am the cofounder and CEO of miLab, a Boston-based ... talented biomedical engineering team from MIT.   I would like to share my own experiences founding and...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur ... ideas than mediocre people with excellent ideas. I am lucky to have launched miLab with two close friends... 50 company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building...miLab.  Although many emails resulted in no response, I was still able to get through to a number of experts...

...area, a line about the research I will be doing, and a line about what I hope to discover. This section.... Success breeds success and I have certainly found that the experience I gained in writing small travel... or professor becomes an overnight success. Below I have detailed some tips to improve your grant writing...off, and perhaps the most important bit of advice I can suggest, is to give yourself plenty of time when...when writing a fellowship proposal. Yes, yes, I hear you saying “We know this already!” But, you do need...unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your...helpful for them to know what we are doing and why. I always ask kindly friends and family to read my non-expert...

...universities, and medical schools. I will emphatically deny that I have a magic formula to guarantee a... explain what I was proposing. The result of my test was humbling. My conviction that I had a flair for...anecdotes, and insights. After I wrote the first version of my manual, I added new chapters to incorporate...original blog post below! --Ed.  Eight years ago, I decided to write a "how to" manual on applying for...distill a one-size-fits-all recipe for success. What I can promise is that my suggestions can help you make...think you can accomplish. No matter how many times I say this, candidates regularly try to convince me ...She would then look at me quizzically and ask why I didn't just say that? Bottom line is to appreciate...

...strong. There are a few women that I see at the gym every day. If I skip, I get a bunch of text messages asking... take larger goals (e.g. Figure out what I want to do after I get my PhD) and break them down into smaller...progress. To illustrate this point, consider that I try to exercise every morning, but the temptation ...on setting SMART goals using a Development Plan template. A Development Plan or mentoring journal will...perhaps, asked someone to support your career development as your mentor. How do you make the most of this... reap rewards in the form of reaching career development goals. Check out Joanne's Reddit AMA Add a ...