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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 111 results for: prime editing

Showing: 41 - 60 of 111 results

  1. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate... CRISPR Base Edit CRISPR Plasmids: Base Edit Browse...generate single base pair edits without double stranded breaks. Cytosine base editors are created by fusing...precisely editing DNA bases without inducing a DNA break. CRISPR...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...can convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently...between Cas variant and deaminase to adjust the editing window, or adding fusions that increase product...

  2. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  3. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI... The transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless", as the transposon.... CASTs are most often used in bacteria genome editing, though some mammalian systems have been developed...

  5. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  6. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  7. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  8. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...homology to the DNA flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template...

  9. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  10. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ... class of genome edit. Knock-in mutations and gene editing Targeted genome editing events fall into ...HDR events over other edits Like I said earlier, HDR is not the most common editing pathway for DNA repair...Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells Resources... these types of mutations, making it an ‘easy’ editing job to get done. However, this is not the case ...room for error. It’s no surprise then that these edits take more planning and finesse then their knock-... a gRNA to where you would like to introduce the edit. Finally, you must stipulate what sequence to introduce... coding region of a gene, make sure that the PAM edit you introduce is a silent mutation, so you don’t...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...maybe you’re performing a single base pair edit with a prime editor. What next? When generating a knock-in...validating your edit, it’s important to know a few things about your target cells and the desired edits. First,... WT and edited cell lines. Then decomposition analysis is performed yielding a graph of edits in the input...flanking the editing site on either side of the amplicon. Based on the frequency of out-of-frame edits, you ...digestion, indicating targeted edits.   Interested in this method, but your edit doesn’t affect any endogenous...can introduce a silent, passenger edit to serve as a proxy for your edit of interest. Using the same gRNA... precisely estimate editing frequency in a bulk population and also identify edits in individual clones...

  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  13. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Activation Barcode Knockout Inhibition Base Editing Prime Editing Other Species Human Mouse Fly Yeast T. gondii... 227706 227707 227708 Prime Editing Human Human Mouse Adamson 3rd 1-20 per edit Varies CRASP-Seq Gene ...3rd ~7 11,364 Dow Lab Base Editing Sensor Libraries 179217-179219 Base Editing Human, Mouse Dow 3rd ~100...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...46,354 BARBEKO sgRNA Library 174163 Knockout, Base Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus...95,893 CRASP-Seq BE Tiling Library 232070 Base Editing Human Gonatopoulos-Pournatzis 3rd Varies 8,594 ...

  15. CRISPR Plasmids and Resources

    Type
    Collection
    ...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation...nickase enzymes generate single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...

  16. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template...

  17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...In this and upcoming posts in this optogenetics primer series, I’m happy to share some of the tips and...driver mice. Current protocols in neuroscience / editorial board, Jacqueline N Crawley  [et al]. 2012;Chapter...approaches. Current protocols in neuroscience / editorial board, Jacqueline N Crawley  [et al]. 2013;4(435...

  18. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...CRISPR genome editing has quickly become a popular system for in vitro and germline genome editing, but in ...packaging limit. For CRISPR-based RNA editing, the REPAIR (RNA Editing for Programmable A to I Replacement... to deliver larger cargo such as base editors and prime editors. While using AAV to deliver large transgenes...in vivo gene editing approaches have been limited by problems with Cas9 delivery. Adeno-associated viral...studies. One of the pitfalls of CRISPR/Cas9 genome editing is the potential for off-target effects. To compare...AAV8. Since the efficiency of CRISPR/Cas9 genome editing varies across targets, they tested two genes in...proteins. The Sontheimer lab used NmeCas9 with AAV to edit two different disease-causing loci in mouse liver...

  19. CRISPR Plasmids - Yeast

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine to inosine...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template...Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase...used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid Description...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...engineered prime editors to expand their PAM flexibility. Find the engineered prime editors here A lentiviral... variant (microABEI744) has improved on-target editing efficiency and reduced RNA-off target footprint...pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to...discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of huge phages. In this... Scientists looking for more compact genome editors due to delivery constraints and scientists seeking...
Showing: 41 - 60 of 111 results