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We narrowed to 130 results for: primer

Showing: 41 - 60 of 130 results

  1. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... the tenth day of CRISPR, Addgene gave to me: a primer on prime editing.  On the eleventh day of CRISPR...

  3. The Twelve Days of CRISPR

    Type
    Blog Post
    ... the first day of CRISPR, Addgene gave to me: a primer on prime editing.  On the second day of CRISPR,...

  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...by homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...versions of the toehold switches and amplification primers. This helped make sure that we had a number of ...assay’s capabilities. Specifically this includes primer directed isothermal RNA amplification, new toehold...

  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... in their own lab.  If depositors have provided primer sequences for use, these are listed.  We also provide... provide a link to our list of common primers used for sequencing. Growth in Bacteria: This section describes...

  10. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...determined by a standard curve of known quantity and primers specific for a viral DNA sequence. *Pro-Tip*: Ad5... Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of CRISPR/Cas9...

  11. Antibody Production

    Type
    Collection
    ...residual plasmid is extracted and amplified with primers targeting the heavy chain and/or light chain variable...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...sequence, and an extension sequence containing a primer binding site and a reverse transcription template...length of the RTT overhang, the GC content of the primer binding site, and many more. Once we had our datasets...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...suitable for PCR amplification using pre-designed primers. By using this library to infect an animal expressing...from the infected animal and use the pre-designed primers to determine which version(s) of the capsid gene...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...the target site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an ...

  15. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...for proteins required for strand invasion and a primer for DNA repair synthesis. The ssDNA strand then...invade an intact homologous template to serve as a primer for DNA repair synthesis, ultimately leading to...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...activity, enhance binding between the template and primer binding site, increase processivity, or improve...editor; RTT = reverse transcriptase template; PBS = primer binding site. Image from Doman et al., 2023, under...containing the reverse transcriptase template and primer binding site. If this tail is degraded, the truncated...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...by homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template...

  20. Protocol - How to Design Primers

    Type
    Protocol
    ...folding. One also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification... Protocols Designing Primers How to Design a Primer You may also like...Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers are necessary when running a...ends of the hybridized primers must point toward one another. The size of the primer is very important as...hand, a long primer is used to amplify a eukaryotic genomic DNA sample. However, a primer should not be... be too long (> 30-mer primers) or too short. Short primers produce inaccurate, nonspecific DNA amplification...temperature (Tm) of 50-60°C Primer pairs should have a Tm within 5°C of each other Primer pairs should not have...
Showing: 41 - 60 of 130 results