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We narrowed to 216 results for: rras

Showing: 41 - 60 of 216 results
  1. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ...http://en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712...CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan; 339 (6121): 819...
  2. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ...to explore this role, how to seek out such an arrangement, and how to operate once in that position. What... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...
  3. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ...produce functional R-mAbs. It was recognized that an aberrant IgG light chain transcript was expressed by some...the restriction enzyme BciVI was present in the aberrant light chain sequence, but was predicted to occur..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG ...
  4. Plasmids 101: Modular Cloning

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    Blog Post
    ... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering them to these...start from scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, ...
  5. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post
    ...per each dCas9, SunTag uses a repeating peptide array to fused with multiple copies of VP64. By having... histone acetyltransferase domain capable of rearranging chromatin structure. This method has shown higher...Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation...
  6. An Introduction to Adenovirus

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    Blog Post
    ...transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Zhou, D., Lin, S. W., Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl...
  7. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post
    ... the past few years, scientists have created an array of base editing reporters that can help you do just...successfully used GO to induce the translation of an array of different reporters including mScarlet-I, luciferase...initiate protein expression, it can be used for an array of detectable proteins including fluorescent proteins...
  8. Five Popular Model Organisms, Part 2

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    Blog Post
    ...2009). Rodents have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016...biology tools to generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed...in research (Krämer, 2015). As a result, a vast array of molecular tools, such as gene manipulation and...
  9. Five Popular Model Organisms

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    Blog Post
    ... has become an ideal model organism to study an array of topics including development, genetics, and the... A huge advantage of using the fruit fly is the array of genetic tools, such as the GAL4/UAS and LexA ...organisms. Worms have been used to investigate an array of biological systems and processes including the...
  10. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...so that at least one might be useful in a broad array of pathogenic Proteobacteria. We placed the toxin..., Shigella flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions...development of allelic exchange vectors will produce an array of great options, and continue to improve the efficiency...
  11. Synthetic Biology - Overview

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    Collection
    ... Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin O'Shea Martin Parniske Antonio Richart Herbert...
  12. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11...reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12...be used to visualize translocations, genome rearrangements and cancer-associated chromosome shattering...subpopulation of mitochondria is selectively labeled by irradiating the photoactivatable FP, then the overlap between... MYC, Flag), they can be deleterious and cause aberrant protein folding, expression, or even affect cell...
  13. Viral Vector Guides and Plasmids

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    Collection
    ...viral production and transfer plasmids for a wide array of research purposes. Interested in Addgene's viral...
  14. 8 Great Depositor Research Stories

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    Blog Post
    ... depositors for sharing your science with us! Corralling and Cornering the Goldilocks Antibody: Guest ...drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen...
  15. Three Tips to Organize Your Lab Notebooks in the New Year

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    Blog Post
    ...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...
  16. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post
    ...supporting your research, Addgene is creating an array of educational materials to accompany our antibody..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of...
  17. The Strength of Story Telling

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    Blog Post
    ...Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your work The part...programmed to appreciate and understand. We build narratives around the self, too — when talking to friends...
  18. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post
    ...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well ...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...
Showing: 41 - 60 of 216 results