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We narrowed to 91 results for: sas;

Showing: 41 - 60 of 91 results

  1. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...called evoCAST — an evolved CRISPR-associated transposase (CAST). Back up a second. What are CASTs? CASTs...Bacterial genome engineering using CRISPR-associated transposases. Nature Protocols, 19(3), 752–790. https://doi.org... with a laboratory-evolved CRISPR-associated transposase. Science, 388(6748), eadt5199. https://doi.org...

  3. CRISPR Plasmids and Resources

    Type
    Collection
    ...generates large genomic deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional...

  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...along with a helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest...flanked by terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, transgenes...transgenes can be removed by re-expressing the transposase. A challenge of using PB to generate iPSCs is that...

  5. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...exchanges bound GDP with GTP, resulting in the disassociation of the Gα subunit from the Gβγ dimer, activating... in response to the ligand, the complex will disassociate, resulting in a decreased GFP2 emission. The...

  6. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...protein (HRP), primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it...of signaling molecules. Assay Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...exchanges bound GDP with GTP, resulting in the disassociation of the Gα subunit from the Gβγ dimer. The Gα...References and resources References Kroeze WK, Sassano MF, Huang X-P, Lansu K, McCorvy JD, Giguère PM,...

  8. Antibodies 101: Conjugation

    Type
    Blog Post
    ...lends itself well to assays like western blots and ELISAs. Chromogenic reactions produce a color change, ...immunohistochemistry, though they are also a common choice for ELISAs.  Biotin Biotin is used in a range of antibody-...

  9. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

  10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Types of ELISA There are four different types of ELISAs, which differ in the way the antigen is detected...available for your antigen as the other types of ELISAs require two different antibodies. As mentioned ...

  11. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...bypassed by leaving out Cas3 — a tactic some CRISPR transposase systems utilize with type I-F Cascades. ...Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano...

  14. CRISPR Guide

    Type
    Guide
    ...GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp...Tn7-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions...recruits the transposase proteins to the target location in the genome, where the transposase then carries...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases..., and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems like piggyBac or Tn7 allow... elements into the genome. CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) ...) combine the large-scale capacity of transposases with the precise DNA-targeting of CRISPR-Cas systems...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes... optogenetic tool for localized, microtubule disassembly. Current Biology, 32(21), 4660-4674.e6. https...

  16. Typing CRISPR Systems

    Type
    Blog Post
    .... Type I systems are also employed as CRISPR transposases, by leaving out Cas3. Type I is further subdivided...paradoxically called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA...

  17. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...in the experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein...

  19. CRISPR Guide Update

    Type
    Blog Post
    ...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe...
Showing: 41 - 60 of 91 results