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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 94 results for: sgrna

Showing: 41 - 60 of 94 results

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting the guide...pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance from...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

  7. Cre-lox system

    Type
    Collection
    ...60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS... Zhang 60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR Cre and sgRNA coexpression Cbh AAV Zhang...ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Cre, KASH-tagged EGFP, and sgRNA expression...expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral Jacks 60877 pMAZe...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... the Zhang lab created an all-in-one SaCas9 and sgRNA construct using the liver-specific serotype AAV8... a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome modification, but they...

  10. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...trace files to the TIDE tool online along with your sgRNA sequence. The TIDE software will generate a graph...along with the estimated editing frequency of your sgRNA-Cas9 combo. Pro tip: When designing your PCR, ensure...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... plate, co-deliver into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100 ... a typical size (~5 kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate. A good example... a typical size (~5 kb) donor plasmid with Cas9/sgRNAs vector, for a one well in a 24-well plate. A good...

  12. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...lab used single-AAV delivery of Nme2Cas9 and the sgRNA to demonstrate efficient editing in adult mice and...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity...Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised ...

  14. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...of 5%. Your experiment involves making a large sgRNA library to thousands of genes and targeting and ...sequencing millions of cells. For every individual sgRNA, you recover a minimum of 1,000 targeted cells to...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...regulator. The lab expressed both dCas9 and the ctrA sgRNA and saw that the levels of ctrA mRNA decreased after...

  19. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...many software tools predict off-target effects of sgRNAs using computational methods. They identify possible...mismatches based on the sequences of the genome and sgRNA. This is a good starting point for most researchers...
Showing: 41 - 60 of 94 results