Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 53 results for: stem loop

Showing: 41 - 53 of 53 results

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti... 2xFLAG tag in pCS for a variety of systems, including Xenopus HA Epitope tag...ultraID in mammalian cells with the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of ...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...iPSC generation Deriving iPSCs (induced pluripotent stem cells) through the expression of transcription factors...this work are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting...reprogram human fibroblasts into induced pluripotent stem cells. By replacing wild type SOX2 with either a...extra-chromosomal telomeric DNA), which accumulates when T-loops (secondary telomeric structures) are deleted. Reduction...nickases, and gene activation systems as well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression... I CRISPR systems, but only recently have inhibitors for the popular type II CRISPR systems, which include...Gateway-compatiable toolbox for studying the nervous system The Cole lab recently deposited the Tol2 Gateway-Compatible...

  4. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ... is expressed as inverted repeats separated by a loop region that facilitates the formation of a hairpin...‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella patens with an Internal Marker... Morgens DW, Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for... Goshima G (2012) An Inducible RNA Interference System in Physcomitrella patens Reveals a Dominant Role...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...engineering in 2012.  Many of these advances have stemmed from the goal of reducing off-target Cas9 activity...Phillips-Cremins JE (2019) LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods...their CRISPR system. This common optogenetic technique can be employed in other split systems where light...HDR using split systems Later in 2015, the Sato lab unveiled a photoactivatable system to cleave a target...activation using dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light ...Gersbach’s lab at Duke University developed similar systems based on the light-inducible heterodimerizing cryptochrome...proteins. The goal of both groups was to create a system that would use light to turn on and off gene expression...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...regions are often reciprocal, forming a neuronal loop which is activated and strengthened during memory...neuronal communication. Viral vectors and the nervous system With the advent of viral vector technology, new...peripheral site of infection to the central nervous system where it replicates, spreads, causes neurotoxicity...WGA-Alexa Transsynaptic Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic...delivery to the central and peripheral nervous systems. Nat Neurosci 20(8), 1172–1179. PubMed PMID: 28671695...serotypes 1, 2, 5 and 8 in the rat nigrostriatal system. J Neurochem 109, 838–845. PubMed PMID: 19250335... retrograde tracer in the rat brain hippocampal system. Brain Res 152, 145-150. PubMed PMID: 79432. Schwab...

  7. CRISPR Guide

    Type
    Collection
    ...Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID... Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular...enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic edits...Cas9 (CRISPR Type II) systems, while AcrV inhibits Cas12a (CRISPR Type V) systems. There are rare cases...The two main classes of CAST systems are type I and type V-K. Type I systems use a type I Cas effector, ...), while type V-K systems use a type V-K Cas effector, Cas12k. No matter the system, the Cas effectors...-vector systems, in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which...

  8. CRISPR Guide

    Type
    Guide
    ...Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID... Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular...enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic edits...Cas9 (CRISPR Type II) systems, while AcrV inhibits Cas12a (CRISPR Type V) systems. There are rare cases...The two main classes of CAST systems are type I and type V-K. Type I systems use a type I Cas effector, ...), while type V-K systems use a type V-K Cas effector, Cas12k. No matter the system, the Cas effectors...-vector systems, in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which...

  9. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...Expression of this system is tunable via a rhamnose-inducible promoter. While this system was optimized for...developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for ...for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com aptamer which binds... This editor still retains A-to-I editing. This system is called RNA Editing for Specific C-to-U Exchange...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...cholerae that encodes a Type I-F CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA...eukaryotic genomes.  Components of a transposon system Transposon: The transposon is the DNA sequence...take a closer look at three different transposon systems adapted for use as research tools: Sleeping beauty...otherwise, piggyBac was discovered in the cabbage looper moth (Potter et al., 1976). Its target site is ...ve learned about some of the popular transposon systems, let’s take a look at how they can be used in the...Transposable Elements by Guide RNA-Assisted Targeting) system. INTEGRATE is based on a naturally occurring Tn7...transposon end sequences (Fig. 3A).  For the INTEGRATE system to transpose, first the QCascade complex surveys...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ...calcineurin B homologous protein 2 - CHUK conserved helix-loop-helix ubiquitous kinase IKBKA, IKK-alpha, IKK1, ...infection depends on two systems that work in concert: the innate and adaptive immune systems. Upon infection,... Lentiviral Expression (MuLE) System Kit : A modular and flexible system that can be used to construct...infection, the immune system triggers several processes to activate cells to recognize and get rid of infectious...following exposure to a pathogen. The innate immune system is not specific to any particular pathogen. It ...Cytokines are molecules that enable cells of the immune system to communicate with each other across long distances...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Halo CBA ALS Marvin Bentley 134901 pUC19-hAPOE-R-loop APOE T7 Alzheimer's Andrew Deans 137187 pAAV FLEX... Multisystem proteinopathy, ALS Nicolas Fawzi 98667 hnRNPA2_LC_WT_S329C HNRNPA2B1 His T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy... T7 Multisystem proteinopathy, ALS Nicolas Fawzi 157865 hnRNPA2_RRM1 HNRNPA2B1 His T7 Multisystem proteinopathy... Dementia Joan Massague 58260 pBabe-Neuroserpin oloop SERPINI1 Dementia Joan Massague 58261 pBabe-FLAG-Neuroserpin...

  13. Modular Cloning Guide

    Type
    Guide
    ...Biology Plasmids Modular Cloning (or MoClo) is a system for efficiently assembling multiple DNA parts into...assembling gene-editing tools, and more. The MoClo system consists of three sets of cloning vectors (Level...coding sequences, CRISPR parts GreenGate Cloning System Plant Expression Jan Lohmann 56 plasmids to create...Maizel 68 plasmids to extend the GreenGate Cloning System (Lohmann) with additional Level 0 modules and components... plasmids compatible with the GreenGate Cloning System (Lohmann) to create plasmids with multi-gene constructs...MoClo-YTK parts for use). Yeast GoldenBraid Cloning System and Toolkit Yeast Expression Elena Caro Bernat ...generating transcriptional units using the GoldenBraid system, for integration in S. cerevisiae . Easy-MISE Toolkit...
Showing: 41 - 53 of 53 results