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We narrowed to 95 results for: t aav

Showing: 41 - 60 of 95 results

  1. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right...C. J., Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza...N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J....

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...Bispecific T-cell engagers (BiTE®s), bind CD3 on T cells and a tumor-specific antigen, it brings T cells to...chimera of scFvs and T cell receptors and are expressed on T cells to make CAR T cells. The extracellular... allows for their delivery by viral vectors like AAV, which could be useful for delivering scFvs that ...recognizes an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal...transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv that binds cancer-related...binding of the scFv part of the receptor can activate T cells to kill cancer cells. scFv’s small size also...Hardman K, Jacobson J, Johnson S, Kaufman B, Lee S, Lee T, Pope S, Riordan G, Whitlow M (1988) Single-chain ...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... of the toolkit in action, see Cermak T, et al. 2017. Cermak T, et al. Plant Cell. 2017. PubMed PMID:...interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally manipulate...tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here...27798629 Listen to Our Podcast Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that...extra-chromosomal telomeric DNA), which accumulates when T-loops (secondary telomeric structures) are deleted...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ... ideal for size-limited AAV delivery. PE6c and PE6d are optimized for dual-AAV systems. These variants...gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying the production...process and could even enable the creation of CAR-T cells directly within the body. Overall, DIRECTED ...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...APEX targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression...sgRNA TetR H1-2O2 Nathanael Gray 35625 pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA...promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator...promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On vector with neuron-specific expression...Plasmid #27106 for rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG... Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...

  6. What the HEK?

    Type
    Blog Post
    ...Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ... the most commonly used line expresses SV40 large T antigen and is thus dubbed HEK-293T. Given that the...viral production because they express the SV40 large T-antigen. This allows them to replicate viral plasmids...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed targets. ...from Neumann et al. 2024. The team showed that AAV-delivered CHARM could silence the expression of prion... (2024). Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic...Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M., Mercer,... CHARM plasmids here!   Neumann, E. N., Bertozzi, T. M., Wu, E., Serack, F., Harvey, J. W., Brauer, P....

  8. AAV for Neuronal Tracing

    Type
    Collection
    ... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA...deletion-mutant rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging...to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all the viral genes...

  10. Plan Your Experiment

    Type
    Collection
    ... collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector...delivery. Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity... transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host...also frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the...blog post on overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged...Marjanovic, N. D., Dionne, D., Burks, T., Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024)...Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions...for the first time (Figure 3). The team also used AAV9 to deliver ASAP5 to the cortex and imaged voltage...

  12. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_...Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown...Sontheimer, E. J., Goodale, A., Greene, M. A., Green, T. M., & Doench, J. G. (2024). Modular vector assembly...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc...Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout...William Hahn, David Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark...Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James Wilson...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids...Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...glycoprotein along with the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA...example, LDLR expression in hematopoietic stem cells, T cells, and B cells is low. Infection of these cells...Liong SH, Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo...nmeth999 Wickersham IR, Lyon DC, Barnard RJO, Mori T, Finke S, Conzelmann K-K, Young JAT, Callaway EM (...

  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming the surface..., F., Nalin, R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence.... L., Veseli, I., Moore, R. M., Kiefl, E., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B.,...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...to calculate the infectious titer: $$T = {V*C*D\over v}$$ Where: T = Infectious titer, TU/mL V = Viruses...Titration Assay Lentivirus Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...systems have enzymes small enough to fit into a dual AAV system, albeit with lower knockdown efficiencies....HITI and HDR. PASTE is also compatible with both AAV and adenovirus templates. The system, which is larger... 6. PMID: 34489594. Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski,...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...occasions, shRNAs are transiently expressed using either AAV backbones or species-specific expression plasmids...8674(04)00045-5 De Rie, D., Abugessaisa, I., Alam, T., Arner, E., Arner, P., Ashoor, H., Åström, G., Babina...Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated... D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short hairpin RNA (SHRNA): Design, delivery...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that...also makes it suitable for multiplexing and use in AAV. Scientists have also developed CRISPR editing technologies... Biotechnol . 32(3):279-84. PMID: 24463574 Fujita T, Fujii H. 2013. Efficient isolation of specific genomic...A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci...Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. 2016. Multiplexed labeling of genomic loci with dCas9...Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. 2014...specificity. Science . 351(6268):84-8. PMID: 26628643 Wang T, Wei JJ, Sabatini DM, Lander ES. 2014. Genetic Screens...

  20. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing...across four different types of human cells, including T cells and hematopoietic stem and progenitor cells....
Showing: 41 - 60 of 95 results