We narrowed to 653 results for: EPO
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TypeBlog Post...they are integrating viruses – they physically deposit their cargo into the host’s DNA. These viruses ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just...
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Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...Associate Professor at Boston College. National reports stress the importance of providing authentic research...Students have used these plasmids, which have been deposited with Addgene, to successfully analyze Met protein... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo... -
Tips for Technical Support Calls
TypeBlog Post...the experiments performed by our thousands of depositors. That means their original articles are your ... Addgene Help Center Get Help with the Addgene Deposit Process Resources on Addgene.org Check Out Our... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...supervisor might expect weekly or biweekly progress reports. However, a hands-off busy supervisor might get...update, and whether he or she would like a written report or to meet in person. Regardless of your supervisor... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927.... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods for various...or cell lines, please consider sharing them by depositing with Addgene! Figures were adapted from: Hall... -
3D Printing Meets CRISPR Cas9
TypeBlog Post... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...