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.... However, most lesioning methods destroy areas larger than intended and may unintentionally remove connecting...an animal’s movement in an open field box or a circular corridor apparatus using a camera or tracking ... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced...time in the open arm and were less risk averse. Similar to the elevated plus maze, the light-dark box is...protected) area. When measuring the time spent in a particular part of the apparatus, it turned out that the...downregulating expression of miR124a achieved a similar improvement in the forced swim test.  Another brain...this further and showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is...

... SPH mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators...activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented...delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was...neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church Lab. Neuron-specific...microglia are a notable exception) necessitates such molecular gymnastics for sensitive applications such as ...screening. The plasmids used by the Qi Lab for this particular application can be found here.  Why CRISPRa? ...well-suited, including studying mRNA dosing effects, cellular reprogramming, and disease modeling. Further, ...

...model organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2 ...In this heyday of molecular biology, many scientists do a lot of DNA work but never get to actually manipulate...materials sharing in their dissemination plans and in particular, that they all share the plasmids created under...which have been used to treat heart disease. Caterpillars that eat milkweed generate cardenolides that... poisonous) for bird predators - birds eat a caterpillar once, vomit, and never do it again. Cichlids ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species... fascinating social behavior. For example, two similar species have very different social behavior. One...

...important regulator of embryonic development The largest conference room at Addgene is named Sonic Hedgehog...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments...contain SHH, you can find them all here! The most popular SHH plasmid however was deposited by none other...transposons as a non-viral system to integrate particular genes into mammalian genomes and easily disrupt...Ivics et al 1997. Spaghetti Squash, a regulator of cellular contraction You may think that a gene called "...the fly actomyosin cytoskeleton that regulates cellular contraction. This network is composed of actin...find the exact origin of this gene name but the larval gut of bagpipe mutants vaguely resemble a bagpipe...

...realized the need to modernize our approach to handle larger volumes of data. In this two-part blog series, ...software was originally designed to help us work in large formats, such as 96-well microplates, and thanks...which existing script(s) have been developed for a singular purpose (ex. adapter trimming, quality score analysis...or script will run and can be thought of as a particular step in the overall pipeline. By specifying the...container, we customized each container for its particular purpose and knew we could rely on the containers...oriented ourselves with the basic concepts and vocabulary of Nextflow so that we could translate our pipeline...concept of channels and implemented the input declaration correctly, the script block in the second process...

...professor displayed a codon table — a chart every molecular biologist has seen before, showing how DNA sequences...into amino acids. And I was astonished that the molecular language shared by every living thing fit onto...different from other plasmids? If we all use the same molecular code, can I transfect any old bacterial plasmid...mammalian plasmids, mammalian vectors are even less similar to bacterial ones. The mammalian analogue of bacterial...oris to drive amplification, which helps increase cellular uptake, but this approach requires compatible ...machinery. Alternatively, some slow-growing cells, particularly mammalian cells like neurons, may not replicate... your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes. For...

...experiencing similar situations to themselves, allowed the participants to feel more comfortable at a large meeting...James from Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...Just like analysis pipelines for DNA that group similar sequences together in bins, we thought that binning...structure and support network for people attending large meetings. A key was to provide each bin with a mentor...-meeting survey indicated that the program was largely a success, but also helped us find areas for improvement...greater effort in the future to match people within similar fields (ASM Microbe has eight tracks), something...despite the fact that it was gray. He has worked in molecular and microbiology for the last two decades as a...

...understanding of the molecular behaviors of chemicals and proteins to learn how a particular drug or medical...novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running the marketing...healthcare consulting firms can be involved in many similar activities depending on the expertise of the senior...challenging. For instance, the client might need to get a large amount of market research done to inform a go/no-go...might outline the impacts of combining targeted molecular therapies for cancer with chemotherapies, or developing... science and the commercial applications of a particular therapy. With your scientific knowledge behind... to a community of colleagues who are pursuing similar jobs and non-academic careers. Now, more than ever...

...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved ... was necessary and reasonable. PLOS has since clarified their data sharing policy and recently announced...manuscripts that had been processed since the declaration, only a small fraction (<1%) of authors have ...their own data, with some smaller labs feeling that larger, better resourced, groups would harvest and reanalyze...efficiently trawled their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel... PLOS’s credit, the organization subsequently clarified what the policy intent was and provided an FAQ...PLOS’ Bold Data Policy - by David Crotty (The Scholarly Kitchen) Read more Addgene blog posts about scientific...

...requested! Fire Lab C. Elegans Kit - Our first popular "kit," Andrew Fire's set of vectors for C. elegans... 2005. shRNA - In 2006, we saw an increase in popularity of empty backbones for shRNA expression, such...plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years. Induced Pluripotent...the most frequently requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to...plasmid. A variety of plasmids continued to be popular years after they were added to the repository, ...activator-like effector nucleases (TALENs). Our most popular kit of the year was the Golden Gate TALEN Kit, ...Plasmids for fluorescent tagging have long been popular with scientists, but 2012 was marked by increased...