We narrowed to 1,056 results for: STI

...Mentoring series will cover 2 of the 7 mentoring questions I set out to answer. First, I will describe some...diligent about replying and thinking about good questions and answers. Professional networking groups will...and move on. If they say yes, be prepared with questions and topics to discuss. The mentee is responsible... needs to be open to change and constructive suggestions and feedback. The best thing to do to make your...

... post was written by Professor Mark Howarth, investigator at the University of Cambridge. His research...the features that would define a good tool for sticking together biological components. Nearly all of ...of us played with LEGO as a child (some of us still do), so we are used to the idea of general interfaces...Biology reactions can add new functionality to existing genes, while being accessible simply by dialing...

...Shaner et al, 2008). This brings us to the key question: what is the best way to compare the photostability...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

..., a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted protein degradation In the Bradner and.... Remarkably, small molecule degraders provide distinct advantages over pharmacological inhibitors including...address your requests for dTAG molecules and any questions to Behnam Nabet ([email protected] fellow at the Dana-Farber Cancer Institute. He is a chemical biologist interested in targeted...

...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic...you will need to make sure that you can easily distinguish each different type of antibody and therefore... through the above steps, you may find that you still have a number of options. If this is the case, you...your assays up and running.  Ed.'s note: More questions on antibody validation? Check out Antibodies 101...

...Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful Cas9 toolbox, Ma ... different fluorescent protein to create three distinct colors. The specificity of these orthologs is ...yellow, cyan, or magenta light. A gRNA with all 3 distinct hairpin sequences produces white light. Created...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...

...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...experienced: wanting to ask and answer experimental questions, but not having the tools needed to do so. Cepko... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found...

...many important questions, areas for informed discussion, debate, and data collection still remain: What...workforce structure? Helping to answer some of these questions, and posing some of their own are the following...grassroots organisations that represent postdocs and institutes that employ them, the diversity of trainee backgrounds...

...funding from a variety of government and academic institutions. New graduate students may assume that their...the mission of the application and the funding institution. Moreover, avoidance of ‘to be’ verbs creates... with the description of the RFA and funding institution. Emphasizing how your solution fits into the ...research project grant application" by the NIH Still want more information on the application process...

...plasmids can lead to far reaching consequences, from wasting time and money on optimizing experiments, to false...all incoming plasmids. If you are interested in requesting one of these plasmids, you can request that it...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...team leaves a note on the plasmid page to make requesting scientists aware of the identified discrepancy...

...and application should be considered The first question you may have is whether a monoclonal or polyclonal... 1998). Consequently, if you find yourself in a sticky species-on-species situation, and other antibody...that users choose antibodies that are easily distinguishable from each other. In a direct approach, that... species or isotype that can be probed with a distinctly conjugated secondary antibody. Consider too that...

...transduced cells and loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes...virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding an inner core which contains...use of this system. New and improved systems are still being developed. A detailed protocol for production...mL of sample. The purity of the sample can be estimated by looking at the ration of DNA (260nm) versus...

...and overlapping complexes that have distinct functions. Many existing methods for identifying PPIs, such...additional interacting factors. Problems with Existing Methods for Studying Protein-Protein Interactions... biotinylated. This makes it’s impossible to distinguish proteins that directly interact with the bait...

...pattern of the antibody binding “distinct imaged pattern.” The distinct imaged pattern should align with...comparison on the antibody signal’s location. If a distinct imaged pattern does NOT align with the atlases...through their own analysis of the background noise, distinct imaged pattern, and exposure times. To test the...

...changeover had no impact on our ability to provide requesting scientists high quality data! Addgene's new ... example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...develop a PCR strategy suitable for the library in question. We will order primers that can amplify the insert...

... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of...pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode...

...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin...box for the “Purpose” field. If you ever have a question about what should go into a cell, hover over the...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...in March 2017 and updated by Angela Holmes and Christina Mork in February 2024.    Additional Resources...

... and a matched isotype control allows you to distinguish between specific and non-specific staining and...norepinephrine, but the recent first-generation tools are still somewhat limited. Following up on their first GPCR-based...session after fear conditioning (D); CS: conditioned stimulus tone. Adapted from Rohner et al. (2026) under ...called TCRAFT: TCR rapid assembly for functional testing. It builds synthetic TCRs in a pooled format, using...

...kinds of CRISPR tools people were depositing and requesting each year (Figure 2). We did a similar analysis...2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests ...continue to develop and share new tools, while our requesting labs continue to request and use new tools. Because... each country. Countries shown are the top 10 requesting countries. FR=France; KR=South Korea; CH=Switzerland...

...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these...models function as thinking tools – eliciting questions like “what does this model representt?" ... "how...creating physical models of proteins that are being investigated in their labs. Soon after we began working with...of the night dreaming about the conformational gymnastics that this protein must undergo as it interrogates...