We narrowed to 1,027 results for: ELL

...happens if you do scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links...moment to let us know what you think, through the yellow popup at the bottom of the page. Happy surfing!...

...include popular envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus...instead, don’t fret; we have backgrounds for you as well! If you use FRET, you might be interested in our...

...plasmids is associated with the "Barcoding cells using cell-surface programmable DNA-binding domains" ...

...multiple plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...

...real career. In art school, I came across Joseph Campbell, and read about his "follow your bliss' mantra...into the arts (or any career to be honest), I would tell them to follow your passion. Do what you love. Don't...

...written content about timely scientific topics, as well as archived seminar presentations. One of SITN'... In return, the public receives trustworthy, intelligible scientific resources. SITN hopes to make DayCon...

...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

... an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role in the company. One ...Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke with amazing people that were...

...mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ...

... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed.  A cell-type specific Cre can provide you with that control...

...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...

...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed...

...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

...upload and share their negative data. After all, they tell me, when something doesn’t work, it’s not a failure...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

... you cite it using both its Addgene ID number as well as the reference for its original publication. To...expressing different types of rhodopsins and sensors as well as references and links to other resources. If ...

..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

...like the cartoon on the right?!? I would love to tell such a dramatic story, but it wouldn’t be my story...career while still in graduate school, you’ll be well suited to follow your passion and land a position...

...isolated resorts creates a sense of camaraderie with fellow attendees from the moment you arrive. Getting off...alone were worth the trip. Dr. Dana Carroll's excellent keynote address was the first of 19 talks given...

... or more “important”, they just need to know you well enough to ask good questions and hold you accountable... can share it with others and get their input as well. Giving a great video presentation is going to be...

...about synthetic promoter AAVs for cell-type expression in retinal cells Resources on Addgene.org Find ...

... region of a different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody...antibodies! References and resources References Liddell, E. (2013). Antibodies. In The Immunoassay Handbook...

...address this, several years ago we partnered with Seqwell to establish a next-generation sequencing (NGS)... small amount of spurious DNA from the packaging cell line, helper and packaging plasmids. We decided ...

... can be used to visualize cells and to quantify targeting of particular cell types. For example, the mDlx...

...industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive...

...again or set up a meeting. If you really got along well, suggest a coffee chat in the near future. Even ...you succeed in any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a...

...Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction...fantasy, pop-sci, true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice...

...hard-to-read sentences.  Grammarly Grammarly is a lot like spell check, but since it’s an internet browser plug-in...managers and productivity tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management...

...skip purification steps and end-repair steps with well-designed fragment overlap sections, while still ...of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires upfront...

...measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the... 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein...

...Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with Addgene causes a paper... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry...