We narrowed to 1,015 results for: LEA

... topic suggestions for our Plasmids 101 series, please let us know in the comments. Note:Marcy Patrick...

...Resources on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR ...

...nervous (I mean I WAS presenting to three department leads, I probably should have been nervous), but I wasn...

...variants of unknown significance – mutations with unclear effects on disease pathology - to determine if ...

...29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The luciferase ...

... to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral service Find optogenetics...

... within the past six weeks. Through Addgene, I learned the basics of lab safety, general work skills —...

...CRISPR-associated.” The most famous of these proteins is Cas9 nuclease, but there are many other Cas proteins as well...

...communicating your research by guest blogging here, just leave a comment if you're interested and our team will...

... Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols...

...suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out Addgene’s monoclonal...

...antibodies may be the right solution for you. Read on to learn more. What is an isotype? Antibodies are composed...

...PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover Download the ...

... research materials as quickly as possible. (To learn more about why MTAs matter, check out another blog...

... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...

...for scientists that helps them share reagents — leading to open, reproducible science,” said CZI Head of...

...targeted genes. In these applications, however, nuclease-dead Cas9 is fused to a transcriptional activator...

...reliable optically evoked neuron activity for at least several months after implantation of fibers, indicating...

...genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of your choice...

... science communication and can use what you've learned to share your science with others.    My favorite...

...I knew anything about being an editor - that I learned on the job. The day-to-day work of a science editor...

...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...

...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

...Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids to all scientists...

...the best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps ...

... the preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite...

...collection.   Check out the SeqWell website to learn more about our plexWell kits and services! Many ...

...what you are capable of. You would be surprised to learn how often I see resumes from good candidates that...

...most detectable and so should be used to tag your least abundant proteins, with the blue and infrared channels...

...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity...Learn about different molecular cloning techniques, focusing on seven common cloning methods. Educational...Restriction Enzyme Cloning Restriction enzyme (endonuclease) molecular cloning is the "classic" cloning ...naturally produced by certain bacteria and archaea, cleave double-stranded DNA (dsDNA) at specific sequence...four to 13 base pairs, and produce predictable cleaved ends in the DNA fragments. Given its prevalence...cloning project. Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can...