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We narrowed to 1,025 results for: URE

Showing: 871 - 900 of 1025 results

  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...lentiCas9-blast to generate Cas9-expressing cells. To ensure that this growth rate effect doesn’t confound your... that express cre recombinase. Typically, this feature is used to attain cell-specific expression in transgenic...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...with each other. Below we will outline the common features as well as the differences within their viral ...Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and Function. Viruses. 2021 13(3):388. DOI:10.3390...

  4. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal vector ...low immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  8. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express your...immediate and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...

  11. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion of keratin...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now...

  13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...here, the SMASh tag does offer a few other unique features. If your protein is not amenable to being tagged...effective it will be, given the diversity of protein structures, interacting partners, and localization. Thankfully...

  16. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

  17. PhD Applications After COVID

    Type
    Blog Post
    ...presentation. It can help soothe your nerves and make sure you cover everything you need to during the interview...students; an online meeting can take some of that pressure off. This question and answer process can also...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...much more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking...knocking the GFP-Puro cassette into the locus (see figure below). The double-MMEJ strategy looks very similar...

  20. How to Be an Excellent Trainee

    Type
    Blog Post
    ... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...printed protocol, or maybe you would like to take pictures/videos of a machine set up while your mentor is...

  23. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...process that will avoid such miscommunications in the future. Assumptions: Making incorrect assumptions can...Resolving conflicts is great, but avoiding unnecessary future conflicts while giving people the tools to resolve...

  25. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

  30. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie...
Showing: 871 - 900 of 1025 results