We narrowed to 1,042 results for: URE

...-life balance. It is harder to manage when the measure of work is not just time spent at the desk, but...place as described by Sebastiano Massaro in his Nature Biotechnology article “Managing knowledge-intensive...

...new. Science involves success and, more often, failure. It’s useful for non-scientists to understand not...science and society 6)   Consider participating in a future SciFund Challenge. While the goal is ostensibly...

...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with...

...lanceolatum in 2012. Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues...protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID: 23524392...

... been difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE...splice acceptor and donor sites, and introducing premature stop codons.    New from the viral service By...

... tool. They can search the database by certain features like serotype, promoter, or tool type and each...use these questions as a guide for designing your future injection experiments. Have your data or lab notebook...

...applications in cellular biology. Van Haren J et al. Nature Cell Biology 2018 Pubmed PMID 29379139. The “...Riboglow platform at Addgene. Braselmann E, et al. Nature Chemical Biology. 2018 PubMed PMID: 30061719. ...

...edit, and I want to make sure that I (and Addgene) don’t forget this in the future. Here’s a list of blog...

...through biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand...map and cartoon below). Including biotin in your culture and inducing protein expression with IPTG results...

...questions help us expand and improve our resources for future users. We’re excited to be distributing new types...fulfilled more than 140 orders for virus! We’ll be sure to keep you updated on our progress and would love...

... "Viral Expression Vectors" chapters, and more pictures and pro tips.  Happy reading! Learn more about...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

...interspaced with linkers that can fold into a secondary structure that can bind specific proteins via aptamer domains...using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

...impact and, in turn, the big picture of the project. For example, how can future regulations impact your work...

... Julie from Novartis who was working with cell culture and who was extremely helpful since she was familiar...useful pieces of information from the workshop. Future workshops for Addgene Addgene strives to be a great...

...? Check Out Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

...synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information in ...resources from the Addgene blog Learn about other features on Addgene's website Find a step-by-step guide...

...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...

...with each other. Below we will outline the common features as well as the differences within their viral ...Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and Function. Viruses. 2021 13(3):388. DOI:10.3390...

...variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal vector ...low immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

... 3. How would you like to select for your flies? Sure, the vector will have an antibiotic resistance gene...differentiation by antagonizing RORγt activity. Nature. 472, 486–90 (2011). PubMed PMID: 21441909. PubMed...

...lentiCas9-blast to generate Cas9-expressing cells. To ensure that this growth rate effect doesn’t confound your... that express cre recombinase. Typically, this feature is used to attain cell-specific expression in transgenic...

...information so you can tap them for advice. As a future alum, these are people you can “cold call” and ...time. Consider this option but be cautious and make sure to choose one that comes highly recommended from...

...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express your...immediate and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed...

...phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy ...gene expression at any copy number in bacteria." Nature biotechnology 36.4 (2018): 352. PubMed PMID: 29553576...

...the plan of being a scientist. I pretty quickly figured out that I just don’t have the patience to be a...Career Channel of the blog so you don’t miss out on future career posts! Additional Resources on the Addgene...

...reporter output was observed in their 293 cell culture system. In cells cotransfected with GFP and the...types of proteins. For proteins with a modular structure, constructing split variants should be relatively...

...spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion of keratin...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now...

... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...

...Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst among...only assist you and make you more qualified for future career opportunities. Becoming involved is not ...

..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...