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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 581 - 600 of 838 results

  1. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... Choosing the promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary... Taxman, D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short hairpin RNA (SHRNA): Design...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...the nuances of the research in as technical of language as possible. Seriously. My suggested litmus test...knowledge that you have given everyone a simple plain language map of where you are going and what you expect...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...

  5. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...techs’ financial books.  To find personnel salary ranges, go to online job information sites (like, indeed.com...focused on research relevant objectives and their tangible resources. For example, if a dean/chair asks “...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... a wide variety of genomic edits — single-base changes, small deletions, and large insertions up to 12... B., Tran, T., … Dimidschstein, J., Fishell, G., Wang, Y. (2024). An enhancer-AAV toolbox to target and...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ..., Ferling, I., Gazda, M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Make one yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can...can I assemble to attack this problem from every angle?” Your science will be better for it. Additional...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...McClinntock who first discovered transposons due to changes in coloration of corn kernels. Aside from corn,... et al., 2021). Target site preference and host range are important to consider when selecting a transposon...

  11. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...retroviral vectors because they target a broader range of cells and their integration into the genome is...you put in your culture. As viral production can change from one batch to another. For experimental consistency...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...service (Lee et al., 2015). This kit offers a broad range of parts, including copy number machinery, chromosomal...elements such as promoters or fluorophores are interchangeable across organisms. When using the MK system,...

  15. CRISPR Guide Update

    Type
    Blog Post
    ... to use CRISPR plasmids in the lab and the vast range of possibilities at their disposal. With this update...

  20. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...fresh media containing selection reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable...cell line. To do this, treat target cells with a range of doses of antibiotic and determine the lowest ...lentiviral aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM complete ...titered your virus beforehand, you can narrow this range according to the results of your titration. Mix ...lentivirus, above) are dying. Perform regular media changes and monitor the growth of the cells. Pro-Tips Depending...culture, so it is important to do regular media changes and maintain optimal growth conditions for the ...absence of cell death, the cell media should be changed every 2–3 days to maintain the dose of antibiotic...
Showing: 581 - 600 of 838 results