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We narrowed to 976 results for: ARC;

Showing: 581 - 600 of 976 results
  1. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...variants, and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many ... & pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor...to Our Guest Blogger! Gal Haimovich, PhD, is a research fellow in the lab of Prof. Robert Singer at Albert...
  2. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post
    ...blogger, Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...Lavis is currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would...
  3. CRISPR Antimicrobials

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    Blog Post
    ... by antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of resistance...’re turning the tables on the bacteria. Future research will show if this new strategy can help us win... on Addgene.org Find CRISPR Plasmids for Your Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design...
  4. AAVs in Retinal Gene Therapy

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    Blog Post
    ...delivered using this vector. For example, although researchers know that Usher Syndrome (a disease that results...encouraging. Importantly, a significant amount of basic research is being done to further improve the technology...Muzyczka N., 2014. AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014...
  5. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post
    ...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...they’re seeking someone who has, to a t, all the research skills under your belt from your last job. You...managing inventories in the lab, or organizing a research project, one can assume that you’ve had to flex...
  6. Getting to Know Expansion Microscopy

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    Blog Post
    ...accessibility (Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural...Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses... microscopy: principles and uses in biological research. Nat Methods 16:33–41. https://doi.org/10.1038...
  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...derivatives are a staple in the lab. GFP is used in research across a vast array of biological disciplines ...protein and facilitating its widespread use in research. Many other mutations have since been introduced... Marcy Patrick...
  8. Plasmids 101: Codon usage bias

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    Blog Post
    ...useful for organisms, they can be problematic for researchers trying to express proteins in heterologous hosts... cost DNA synthesis, one of the primary ways researchers solve the problem of codon choice is to resynthesize...of these factors on their own. Luckily, many researchers have created codon optimization algorithms and...
  9. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...amino acid across an entire polypeptide. Many researchers have created codon-optimization algorithms/resources...they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112...databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292...
  10. Viral Production at Addgene

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    Blog Post
    ...of the transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...purification kits.     Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer... plasmids used in Addgene’s viral production. Barcoding allows our DNA and virus to be monitored throughout...
  11. Plasmids 101: Shuttle Vectors

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    Blog Post
    ...different kingdoms of life. That’s convenient for researchers who want to do experiments in complex eukaryotic...course, that includes you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even...experiments. Reporters Finally, in some cases a researcher may choose not impose selection on cells and ...
  12. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post
    ...yet sometimes overlooked, aspect of scientific research is that the field benefits from collaboration ...neuroscience field is a great example of this; research benefits when the advances of some are shared ...help illuminate (is that pun worn out yet?) your research. Derek P. Simon received his PhD in Cellular ...
  13. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...
  14. Mammalian RNAi Tools

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    Collection
    ...containing other RNAi components, like argonaute, please search our full site . ID Plasmid Vector Type PI Publication...
  15. CRISPR Plasmids - Base Edit

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    Collection
    ...Overview of CRISPR base editing. Browse, sort, or search the tables below for CRISPR base editing plasmids...
  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined...
  17. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post
    ...useful and most importantly, effective. In the research world, there is a myriad of tools this could be...intended target. RNAi is a valuable tool for your research arsenal, and there is still a dedicated field ...about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256...
Showing: 581 - 600 of 976 results