...Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent...

... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell...

...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,...

...find themselves plagued by troubleshooting a virus-based experiment at one point or another. After amplifying...

...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid.  The Addgene Vector Database has more information about common vector backbones...

...Hopefully we will have new tools for plant science based around those technologies in the near future. Science...

...introduction, check out Addgene’s Cre-lox blog post . Based on these Cre-lox recombination principles, scientists... pK170.AAV-TRE-Cre-WPRE (Supernova) TRE-Cre based Supernova TRE AAV Iwasato 85577 pTC-CMV-Tet CreER...

...virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic and...

...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...

...over the other BiFC is a convenient, fluorescence-based method for directly visualizing PPIs in cells. The...

...development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development...

...particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there...

...application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead...

...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone...

...amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online...

...together a really great solution that covered all the bases. You may have also used your writing skills to convey...

...you do? What follows are just a few suggestions based on what I have found to be especially beneficial...

...CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool...

...resources towards the development of a new therapy based on its market need and anticipated commercial success...

...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses...