We narrowed to 710 results for: BASE;
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TypeBlog Post...journal clubs have become popular, including those based on Twitter. At my former academic department, we...
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Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in... -
Academic vs. Industry Postdocs
TypeBlog Post...manager: Unlike academia, promotions in industry are based on people’s ability to manage others and work as... -
Hot Plasmids February 2024
TypeBlog Post...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent... -
Rabies and Neuronal Tracing
TypeBlog Post... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...find themselves plagued by troubleshooting a virus-based experiment at one point or another. After amplifying... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid. The Addgene Vector Database has more information about common vector backbones... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...Hopefully we will have new tools for plant science based around those technologies in the near future. Science... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol... to determine the concentration of your samples based on your sample’s HC + LC area. Example calculation... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...could be harnessed or enhanced for future MagLOV-based tools, just as light-induced conformational changes... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...CREATE method? CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...over the other BiFC is a convenient, fluorescence-based method for directly visualizing PPIs in cells. The... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions... -
Bioinformatics at Addgene
TypeBlog Post...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...