We narrowed to 621 results for: Emb

1. Tips for Using FRET in Your Experiments

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   Blog Post

   ...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf...

2. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   ...excellent efficiency using a commercial isothermal assembly master mix. Introduction of the strong transcriptional...

3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets...

4. Antibodies 101: Introduction to Gating in Flow Cytometry

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   ...running your analysis. In this case, I and other lab members had worked with lymphocytes before, so I knew where...

5. Transferable Skills Guide: Time Management

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   Blog Post

   ...physically cross things out (it just feels good) and I remember things better when I write them down. Some people...

6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...deliver these components to your target cells. Remember, efficient delivery of CRISPR components is essential...

7. Evolution of Lab Techniques

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   Blog Post

   ...smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization of a radioactively...

8. Treating Muscular Dystrophy with CRISPR Gene Editing

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   ...Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi:10.1038/nature.2015.18947...

9. Viral Vectors 101: AAV Variables That Matter

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   Blog Post

   ...Attribution License. Within the nervous system, remember that some serotypes can be transported retrogradely...

10. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ...this might be frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed...

11. Plasmids 101: Dimers and Multimers

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    Blog Post

    ...control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which cannot distinguish...

12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post

    ...recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts used...

13. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ...Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical School/Brigham and Women’s Hospital...

14. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post

    ...undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning...

15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ...handling cell lines. Avoid discussion with other lab members, sneezing or coughing in the lab. The human mouth...

16. Finding Your Science Policy Path

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    Blog Post

    ...backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical year or transition...

17. Quick Guide to All Things Lentivirus

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    Blog Post

    ...liposome) or electrical (electric current forming membrane pores) transfection. These techniques are still...

18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post

    ...perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ... cell mobility are essential processes during embryonic development. These are required for a growing ...

20. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...screen. Currently, eleven published libraries are embedded. These were kindly donated by six different research...