We narrowed to 802 results for: Eng
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TypeBlog Post...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (2013): 823-826. ...
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Five Popular Model Organisms, Part 2
TypeBlog Post... perfect for molecular cloning and metabolic engineering. The E. coli genome has an extensive research...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs... -
Site Directed Mutagenesis by PCR
TypeBlog Post...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire... -
Hot Plasmids: Spring 2025
TypeBlog Post...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...on. In addition, researchers have created many engineered serotypes by mixing, matching, and modifying ...summarize briefly: AAVrg is derived from AAV2 and engineered to infect neurons and undergo retrograde transport... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post..., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L..... E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork... comparison can be very helpful in uncovering strengths and weaknesses of the options. Use this blog as... -
Antibodies 101: Validation
TypeBlog Post... signal, so you’ll need a range of expression strengths to be confident in your data. This is a good option...9. https://doi.org/10.1038/518027a Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS,... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering Read about the Sleeping Beauty DNA transposon... -
Transferable Skills Guide: Teamwork
TypeBlog Post...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...a team is a really good way of creating and strengthening those social bonds. So never mind how much better... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...Creating epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed ...representing a protein super family). To overcome these challenges, we set out to develop a hassle-free, highly ... -
A Case Study of Addgene Bioinformatics
TypeBlog Post...our pipeline. In it, they break down the unique challenges of our platform and how we were able to address... -
Help Us Name Blugene’s New Virus Friend
TypeBlog Post...make-over Get a how-to guide on the new Addgene search engine ... -
CRISPR Guide Update
TypeBlog Post...the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements and enhanced... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog Post... X, formerly Twitter. If you are interested in engaging with us on social media, you can find us on Bluesky... -
The 12 Days of CRISPR
TypeBlog Post...the 1st day of CRISPR, Addgene gave to me: An engineered prime editor with enhanced editing efficiency... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...Imaging of Gene ExpressionAuthors: Marjorie Buss and Mengtong "Tom" DuanVisualizing Your Plasmid Request Data... -
#Deckthelab Contest Winners 2017
TypeBlog Post...used tip boxes for a lab supply tree and set a challenge for her lab mates to create the best-recycled ... -
Bringing Renewable Energy to Addgene
TypeBlog Post...from renewable energy projects right here in New England- the majority of which are solar, wind, or hydroelectric...