We narrowed to 918 results for: IRE

...biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular biological...PCR. We hope these plasmids will help Dr. Duedu inspire new Ghanaian scientists to continue to do excellent...

...insert are often denoted by "wt" either directly before or directly after the insert name.   Example: If ...

...Cambridge before spending time in biotech screening direct interactions between drugs and promoters of oncogenes...and extremophiles for biotechnology and has been directly involved in supervising many successful iGEM undergraduate...

... an introduction to Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with...

...cups of coffee would be consumed throughout the entire 5 day event. Though, to be fair, I think this is... a day, and I had colleagues who were much more wired. Here at #SfN14 there are thousands of posters being...

...target sites 5' of the 5'NGG3' PAM sequence as directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is ...stand breaks and may better facilitate homology directed repair. You can find all of these variants, as...

...about what's out there.”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities...my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew very early...

...sequence of ssDNA. Unlike Cas9, Cas14 does not require a PAM sequence. In addition to this cis cleavage...Cas12 are for the other types of nucleic acids and required a certain sequence specificity in the middle of...

..., and almost all universities and institutions require them for material transfers. When Addgene entered...and emails it straight to you, or we can send it directly to your institution’s MTA coordinator. Organizations...

...still gets requested! Fire Lab C. Elegans Kit - Our first popular "kit," Andrew Fire's set of vectors for...

...otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled by a neuron...gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest makes ...

...smartphone apps you can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ...

...member of the Addgene Advisory Board, and Associate Director of the Genetic Perturbation Platform at the Broad...blogger, John Doench! John Doench is the Associate Director of the Genetic Perturbation Platform at the Broad...

...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

...of newspapers. Science is now being adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist...The other thing photography has shown to me - it requires an awful amount of skill,  time for which most...

...smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and file ... to the necessary time and resource investment required to change the status quo. But with recent improvements...

... been difficult to study without biosensors to directly visualize their activity. In 2019, the first generation..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

...is key: due to differences in the PAM sequences required by each ortholog, a gRNA designed for one dCas9...fluorescently labeled dCas9 isoforms are efficiently directed to the proper target sequence. They were also ...

...RNAi, the field for which Craig Mello and Andrew Fire won the Nobel Prize. What did Dr. Doudna think was...gRNA and crRNA could be combined into one RNA to direct cleavage the result was a simpler 1 protein - 1...

...acids are essential, which means that the body requires them but cannot produce them. By using yeast integration...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...