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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 937 results for: KIN

Showing: 581 - 600 of 937 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... I’m not going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs...the viral particles of the AAV serotype you’re working with are primarily found in the culture media, .... If you’re new to creating gradients, practice making the iodixanol layers before doing your first purification...

  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...fluorescent protein should I use?" And if you're looking for more fluorescence microscopy tools and techniques...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...does not do). Furthermore, molecular biologists working with vectors are, in most cases, interested in ... the viral integrase protein (IN) during step 3 (King et al., 2008) (Hu & Temin, 1990). At step 3, the....1016/0022-2836(73)90277-5. PubMed PMID: 4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C....

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...performing ddPCR Clean everything You must have a clean working area to titer your AAV by ddPCR. Droplet digital...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...available nanobodies from Pleiner et al. Before picking a secondary nanobody, it’s important to review ...expression plasmids here Additional Resources Looking for monoclonal primary antibodies to use with the...

  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...dye, mauvine, was discovered in 1856 by William Perkin. His discovery set off a flurry of activity and...labeling strategies and improved fluorophores are making chemical dyes increasingly attractive and accessible...Medical Institute. He would rather be in the lab making new fluorophores for advanced imaging modalities...

  9. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...enhancer candidates. To use these sequences for making functional genetic tools, enhancers were nominated...Institute are committed to just that, and their painstaking efforts towards screening thousands of enhancer...Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. D., Bishaw, Y. M., Bendrick...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...reliable and robust high quality viral vector service, making an array of ready-to-use viral vectors available.... I would be remiss to talk about them without looking at what they’re delivering and where they’re delivering...of our most popular ready-to-use viral vectors, making up almost 10% of our requests by 2019 (Figure 5A...

  11. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ... replication and regulation in bacteria Before looking at an example plasmid, let's review how E. coli...different ways: methylating newly synthesized DNA, blocking binding sites, or trading out ADP for ATP to re-activate...mRNA and prevent this pseudoknot from folding, blocking replication. Created with BioRender.com. Plasmids...

  12. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...information to explain why you are interested in working for that organization or group. This is particularly... of what to put in your cover letter, start by looking at the requirements in the job description. For... good place to state why you are interested in working for this lab and/or company. Remember to proofread...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...through smart engineering Different FLP versions – making FLP efficient The first version of FLP discovered...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ... Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces Gamma Rhythm and Controls Sensory...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which is analogous...have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating expression... in length and does not require a specific PFS, making it a very flexible cleavage system. LwaCas13a is...

  15. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... of how they actually “report” as biosensors. Making aptamers glow The flexibility of aptamers means...fluorogen and increase its fluorescence, essentially mimicking GFP.  Though the aptamer, called Spinach, required... Eric J. Perkins...

  16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...zebrafish embryo develops outside of the mother, making them ideal for studying vertebrate embryogenesis... target mRNA inhibit its translation either by blocking initiation or splicing. (RNAi, in contrast, engages...unprecedented views into cognitive processes like decision making (Kim et al, 2017). “Brainbow” fish lines have been...

  17. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... delivery to only a few cell types.  If you’re looking for more control in the cell types your lentiviral...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...speech, coordination, purposeful use of hands, and walking. They may exhibit slow head growth, develop seizures...medical journal. Decades later and after years of painstaking work trying to isolate the causative gene for...:2<147::aid-ajmg6>3.0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY (2013) An AT-Hook...

  19. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...BP Reaction takes place between the attB sites flanking the insert and the attP sites of the donor vector...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...fragments, cDNA or Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of...

  20. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... month without a new, drug-resistant ‘superbug’ making headlines in one part of the world or another. ...that only infect bacterial cells), or a bacterium picking up resistance genes from it’s environment (from...resistant bacteria to over-take the population and making them more difficult to treat in the future. Patients...
Showing: 581 - 600 of 937 results