...Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 ...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

...Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the Kotton Lab Learn more ...mission to promote scientific sharing. Listen to learn more about Darrell, his research, and his focus ...

...Attendees consistently mentioned how pleased they were to learn about these exciting scientific topics ... members gave up a beautiful summer Saturday to learn about science, but they didn’t seem to mind! Attendees...

...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. ...something you’d like to see more of? Let us know by leaving a comment or emailing us at [email protected].  ...

...scared of taking risks, afraid of leaping forward but I have learned to push myself to the forefront, ...internship at Addgene. Although my interests are very clear, I had no idea how to translate them into real world...

...spreadsheet of random numbers that’ll leave you, well, #sad. Learn More About CRISPR Pooled Library Screening...PMCID: PMC4744125. Resources on the Addgene Blog Learn to Conduct Genome-wide Screens with CRISPR/Cas9 ...

...Science Signaling in December 2014, he and his colleagues at Duke University, in collaboration with “cloning... of the cancer pathways ORFs kit Wood and his colleagues developed the kit for use in screening for pathways...PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been Used in Cancer...

...scientists standing on the shoulders of their colleagues and all those who’ve gone before them. Given ..."The Secret of Life." 10)     And, last but not least (certainly not for us), Addgene launched this blog... Year, let's make a toast to open science. And, please, share your favorite OA developments of the past...

...openness and trust that comes from caring about your colleagues on a personal level, and employees often cite...project. These projects could be anything from cleaning out the freezers to writing new plasmid protocols... it? Perhaps new ideas or collaborations, or at least a shared sense that we’re not alone in struggling...

...planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI, it’s easy to miss...Starting with a recap of accomplishments, from learning new techniques to finally figuring out a tough...the yearly planning meeting is that the trainee leads the conversation. As the conversation proceeds through...

...and easily. These tagged plasmids also include cleavage sites should one need to remove the tag post-purification...on these and other tags used in the pCri system, please see Plasmids 101: Protein Tags. Beyond E. coli...purification. For a more thorough introduction to pCri, please see the original publication cited below. pCri ...

...some Addgenies. As the gala came to an end, it was clear that the Addgenies had no intention of ending the...tough but memorable one. At the gala, one thing was clear: the sense of family is still the backbone [ba-dum-chh...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

...materials from the Addgene repository. Read on to learn more.  What will be available?  Over 3000 AAV plasmids...AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering process will look slightly... the identity of the packaged DNA and serotype. Please note that Addgene cannot perform functional or ...

...universities and Technology Transfer Offices were, at least, temporarily closing, these groups were willing ... would like to make them available to industry, please reach out at [email protected] and we will...Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19 plasmid collection...

... repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit that controls...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...blog posts Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...

...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading...the IBEX Knowledge-Base is named on the Zenodo release in keeping with their value of shared ownership...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...

...nucleus in space and time, and to understanding how nuclear organization varies across the life of a cell, ...dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has ...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

...those collaborations over the years, he and his colleagues built a Molecular Expressions collection including...much easier for scientists everywhere to get a clearer picture of that intracellular world, even as the...promote the great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson...

...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ... positive selection screening. They and their colleagues including Feng Zhang used the GeCKO library to...CRISPR libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise...