Skip to main content

We narrowed to 840 results for: Met;

Showing: 581 - 600 of 840 results
  1. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ... elucidate cellular structures. Overexpression methods flood the cell with protein, which can both interfere...with BioRender.com. A key feature of our methodology is the use of a donor plasmid that contains long...
  2. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    .... L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to...Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283–310). Elsevier. https:...
  3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The ...transcription of most of the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective...
  4. 6 Tips for Grant Writing

    Type
    Blog Post
    ...these findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas... successful applications, I can't always find something that made them better than my unsuccessful ones...
  5. Tips for Technical Support Calls

    Type
    Blog Post
    ...answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it...a very positive experience, also let us know! Sometimes those few “thank you”s are what get a customer...
  6. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...printer, a laser cutter, woodworking tools, and a metalshop. After two summers in Silicon Valley as a student...
  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...from contaminants using a variety of purification methods. Deciding which expression system to use depends...transiently or through stable cell lines. Both methods produce high protein yields if transfection is ...
  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degron tags can offer over other protein control methods and compare the commonly available types of degron...protein post-translationally, unlike many other methods. Secondly, these tags can be used to manipulate...
  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...these electrical events! Voltage indicators are voltmeters for cells! In this blog we will review how these... described systems. Several adaptations of this method have been to tag, cage, uncage, and trap the chemical...
  10. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...down the question you were trying to answer, the method you used, the main result, and the fundamental ...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...
  11. PhD Applications After COVID

    Type
    Blog Post
    ...speaking, the PhD application process tends to go something like this:   However, as simply as it seems laid...program. An email to a potential PI should go something like this: Introduce yourself, brief educational...
  12. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...receive include the sense that people learned something new? Did the meeting begin and end on time? Did...
  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...it at the time, but gene therapy had fallen - plummeted really -  from grace 2 years prior with the death...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...
  14. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen...viruses and transduction by vectors (which we sometimes still call “infection” as a shorthand, although...
  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...relationship is non-linear and you should use a different method of analysis. Different fields and assays have varying...
Showing: 581 - 600 of 840 results