We narrowed to 660 results for: SIM;

1. Plasmids 101: Optimizing Plasmid Yields

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   ...yields. Why would volume make a difference? In the simplest of terms, more volume = more cells = more plasmid...

2. Experimenting with New Careers while in Grad School

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   ...researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ...

3. Anatomy of a Plasmid Page at Addgene

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   ...depositing lab. There may be other plasmids with similar names in the collection, but you can use this name...

4. Light Sheet Fluorescence Microscopy

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   ...transparent organisms. Advances in tissue clearing Similar to light microscopy, there have been significant...

5. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...organisms with Cas9 risk creating a gene drive? The simplest way to make a gene drive is to insert an endonuclease...

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression...

7. Isolating Genomic Regions of Interest with the CRISPR System

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   ...and RNA sequencing (Fujita et al., 2015). Using similar affinity purification with an anti-Cas9 antibody...

8. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   ...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...

9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...mentioned above, organic solvents can be used to simultaneously fix and permeabilize a sample. But you can ...

10. Summer SciComm Series: Modes of Communication

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    ...or expertise from the start, or if you can begin simply and build up to more complexity if needed. And ...

11. Antibodies 101: Producing Recombinant Antibodies

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    ... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ...

12. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...

13. CRISPR 101: Making a Knock-In Cell Line

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    ...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

14. Droplet Digital PCR for AAV Quantitation

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    ...measure the concentration of multiple targets simultaneously. For AAV titration, you should only need to...

15. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ... from fluorescent proteins given the relative simplicity of the system and the availability of fluorescent...

16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ... 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly...

17. A Career in Grants: How To Become a Grant Professional

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    ..., mid- to late-career grant professionals have similar stories about a time when their passion for an ...

18. Plasmids 101: Protein tags

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    ...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ... experiences in research labs. What are some similarities and differences you have observed as you have...

20. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ... the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...