We narrowed to 729 results for: STO

1. Supporting Rare Disease Research with the 2020 BeHEARD Awards

   Type

   Blog Post

   ...at University of Texas Health Science Center at Houston. Prakash became interested in studying TS when ...

2. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...

3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...

4. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ...science instructor at The Innovation Institute in Boston. When Eugenia is not by the microscope she likes...

5. RNA Extraction Without A Kit

   Type

   Blog Post

   ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

7. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   ...blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender...

8. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   ...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

9. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

10. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    ... 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or the MoBiFC modular bimolecular...

11. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

12. Hot Plasmids February 2024

    Type

    Blog Post

    ... inspire further-improved versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold...

13. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

14. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

15. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    ...colony, as opposed to ordering control mice from a stock center. This serves as a better control since the...

16. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ... in CDG patients are still poorly studied and understood.  In line with this, Prof. Videira and collaborators...

17. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...

18. Lab Automation at Addgene

    Type

    Blog Post

    ...biggest benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different...

19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...