We narrowed to 712 results for: STO

1. Antibodies 101: Choosing the Right Isotype

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   Blog Post

   ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

2. Corralling and Cornering the Goldilocks Antibody

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   ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ... 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or explore the MoBiFC modular bimolecular...

4. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

5. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...colony, as opposed to ordering control mice from a stock center. This serves as a better control since the...

6. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ... in CDG patients are still poorly studied and understood.  In line with this, Prof. Videira and collaborators...

8. Antibodies 101: Stripping and Reprobing Western Blots

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   ...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

9. Progress Towards a PAM-Free CRISPR

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   ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

10. Hot Plasmids February 2024

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    ... inspire further-improved versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold...

11. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...

12. Lab Automation at Addgene

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    ...biggest benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different...

13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...

14. Tips for Titering Your Lentiviral Preps

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    ...significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of...

15. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...patterns in chromatin interactions. The chromatin story told by CAPTURE 2.0 is important, but it’s not the...

16. Common Injection Routes in Mice

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    ...experimental therapies or pain relief, straight to the stomach. This technique involves administering medication...

17. Magnetic Control of Proteins: More than a Dream

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    ...MagLOV. Andrew was thrilled to see that MagLOV’s “astonishing” magnetoresponse was clearly visible whether ...

18. Tips to Make the Most of a Scientific Conference

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    ...research, gathered up your data into an exciting story, and are ready to present your findings at a conference...

19. Viral Vectors 101: Voltage Indicators

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    ...a genetic component. The VSD components have historically come from a variety of sources – voltage-gated...

20. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...addition to genome identification, we developed custom open-access Python scripts that can perform a deeper...