We narrowed to 718 results for: STO

1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...

2. Viral Vectors 101: An Introduction to AAV

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   ...-based method for the titration of AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID...

3. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender...

4. All in a Twist: dsRNA

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   ... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ...

5. Transferable Skills Guide: Cross-team Communication

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   ...service,” did he mean it the same way as Bryan, our customer support manager? When Gizela, the aforementioned...

6. Corralling and Cornering the Goldilocks Antibody

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   ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ... 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or explore the MoBiFC modular bimolecular...

8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

9. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...colony, as opposed to ordering control mice from a stock center. This serves as a better control since the...

10. Antibodies 101: Stripping and Reprobing Western Blots

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    ...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

11. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ... in CDG patients are still poorly studied and understood.  In line with this, Prof. Videira and collaborators...

12. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...

13. Lab Automation at Addgene

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    ...biggest benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different...

14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...

15. Hot Plasmids February 2024

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    ... inspire further-improved versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold...

16. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

17. Antibodies 101: Choosing the Right Isotype

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    ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

18. Progress Towards a PAM-Free CRISPR

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    ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

19. Tips for Titering Your Lentiviral Preps

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    ...significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of...

20. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...patterns in chromatin interactions. The chromatin story told by CAPTURE 2.0 is important, but it’s not the...