We narrowed to 613 results for: Set

1. An Introduction to Adenovirus

   Type

   Blog Post

   ...12727116. Borkenhagen, L. K., Fieldhouse, J. K., Seto, D., & Gray, G. C. (2019). Are adenoviruses zoonotic...

2. Isolating Genomic Regions of Interest with the CRISPR System

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   Blog Post

   ... vast amount of data from a single experimental setup. As we’ve seen for many other CRISPR techniques,...

3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology potential...

4. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   Blog Post

   ... Seth Kasowitz...

5. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

   ...useful in gene therapy applications where a distinct subset of the cellular population needs to be targeted...

6. Hot Plasmids: Fall 2024

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   Blog Post

   ...This strain is then transformed with the sgRNA cassette on a plasmid expressing mCherry and resistance...

7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   Blog Post

   ...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...

8. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   Blog Post

   ...fails to upregulate transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188...

9. Fluorescent Proteins 101: Aptamer Fluorophores

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   Blog Post

   ...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

10. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post

    ...the technical implementation of your optogenetics setup (viral expression, efficiency of laser light transmission...

11. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...

12. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...cases, you should validate it for your particular setup. We recommend starting by searching the literature...

13. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ...insertions such as fluorescent proteins or selection cassettes. This class of template should have homology arms...

14. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post

    ...prokaryotic lineages revealed a curious anomaly amongst a subset of methanogenic archea: these organisms lacked ...

15. Evolution of Lab Techniques

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    Blog Post

    ...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...

16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...

17. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...training in the academic research environment is setup to prepare trainees for further work in academia...

18. Viral Vectors 101: Optogenetic Tools

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    Blog Post

    ...Figure 1: Example of an experimental optogenetic setup. A light source delivers light pulses to the subject...

19. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience...

20. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    ...emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with standard confocal microscopy...