We narrowed to 618 results for: Set

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   ...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations...

2. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   ...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

3. Plasmids 101: Protein Expression

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   Blog Post

   ...amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein...

4. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   Blog Post

   ...As we pointed out last year2, delivering a DNA cassette encoding the cas9 gene and single guide RNAs (...

5. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   Blog Post

   ... Seth Kasowitz...

6. Isolating Genomic Regions of Interest with the CRISPR System

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   Blog Post

   ... vast amount of data from a single experimental setup. As we’ve seen for many other CRISPR techniques,...

7. An Introduction to Adenovirus

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   Blog Post

   ...12727116. Borkenhagen, L. K., Fieldhouse, J. K., Seto, D., & Gray, G. C. (2019). Are adenoviruses zoonotic...

8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology potential...

9. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

   ...useful in gene therapy applications where a distinct subset of the cellular population needs to be targeted...

10. QC Sequencing Technologies at Addgene

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    Blog Post

    ...are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...

11. Hot Plasmids: Fall 2024

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    Blog Post

    ...This strain is then transformed with the sgRNA cassette on a plasmid expressing mCherry and resistance...

12. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...fails to upregulate transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188...

13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...

14. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

15. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post

    ...the technical implementation of your optogenetics setup (viral expression, efficiency of laser light transmission...

16. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    ...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...

17. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post

    ...cases, you should validate it for your particular setup. We recommend starting by searching the literature...

18. CRISPR 101: Homology Directed Repair

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    Blog Post

    ...insertions such as fluorescent proteins or selection cassettes. This class of template should have homology arms...

19. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post

    ...prokaryotic lineages revealed a curious anomaly amongst a subset of methanogenic archea: these organisms lacked ...

20. Evolution of Lab Techniques

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    Blog Post

    ...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...