We narrowed to 740 results for: Sis;

1. CRISPR Library Amplification

   Type

   Protocol

   ... on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not suspended...sufficient DNA for use, pool samples, and continue with analysis. “Sufficient” DNA will vary upon intended use....

2. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   ...touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked...

3. 25 Work From Home Tips for Scientists

   Type

   Blog Post

   ...communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ...

4. What Good Citizenship Can Do for Reproducibility in Science

   Type

   Blog Post

   ...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making...

5. Celebrate National Book Month with Addgene

   Type

   Blog Post

   ...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're...

6. The Crispr Babies Saga Shows the Need for Action, Not More Delays

   Type

   Blog Post

   ...report is that when it became clear that this assistive reproductive technique was feasible, three separate...

7. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   ...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

8. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   ...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

9. FPbase: A new community-editable fluorescent protein database

   Type

   Blog Post

   ...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...

10. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    ...., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ...

11. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    ...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

12. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    ...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution...

13. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type

    Blog Post

    ...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

14. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    ...technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,...

15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type

    Blog Post

    ...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ...

16. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    ...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

17. Components of CRISPR/Cas9

    Type

    Blog Post

    ...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

18. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

    ... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

19. Management for Scientists: Delegating is Key

    Type

    Blog Post

    ...new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage...

20. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    ...current and future features being built for the analysis software program, and meet with the automation...