We narrowed to 738 results for: Sis;
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TypeBlog Post...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...
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25 Work From Home Tips for Scientists
TypeBlog Post...communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...report is that when it became clear that this assistive reproductive technique was feasible, three separate... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... -
Celebrate National Book Month with Addgene
TypeBlog Post...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ... -
Pushing the Limits of DNA Assembly
TypeBlog Post...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of... -
Components of CRISPR/Cas9
TypeBlog Post...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities... -
Management for Scientists: Delegating is Key
TypeBlog Post...new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage... -
CRISPR Library Amplification
TypeProtocol... on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not suspended...sufficient DNA for use, pool samples, and continue with analysis. “Sufficient” DNA will vary upon intended use.... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene... -
Viral Vectors 101: Biosensors
TypeBlog Post... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...