We narrowed to 731 results for: Sis;

1. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   ...technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,...

2. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   ...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   ...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

4. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   ...touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked...

5. What Good Citizenship Can Do for Reproducibility in Science

   Type

   Blog Post

   ...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making...

6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   ...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

7. Components of CRISPR/Cas9

   Type

   Blog Post

   ...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

8. Addgene Welcomes John Doench to the Advisory Board

   Type

   Blog Post

   ... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

9. Management for Scientists: Delegating is Key

   Type

   Blog Post

   ...new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage...

10. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    ...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...

11. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    ...involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu group at the University ...

12. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    ...2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike...

13. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...chromatin-related plasmid in a collection that grows on a daily basis, the lists below provide a snapshot of relevant ...

14. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    ...present, YFI is inactive, preventing repressor cI synthesis and allowing reporter gene transcription to take...

15. A Novice's Perspective on Neuroscience - SfN2017

    Type

    Blog Post

    ...seem to require some mathematical know-how for analysis. If you’re looking to get into neuroscience and...

16. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    ...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset...

17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    ...polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene...

18. Hot Plasmids - May 2022

    Type

    Blog Post

    ... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific...

19. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    ...validate your new construct by Sanger sequence analysis. But for this research, it was likely that the...

20. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    ...data in a manner that demands high accuracy and consistency?  If the answer to any of these questions is...