...and accessibility, more researchers have been utilizing full plasmid sequencing services to verify the...

...Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile Expression System for Drosophila. Methods Mol ...

...designs, we have observed that certain GRSMs may still not perform as expected, perhaps due to idiosyncrasies...

...Conway AE, Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and...

...hear too many science trainees get warnings and still think “but that won’t happen to me, I have a backbone...

...common problems that many others have faced yet, still, this challenge did not hinder them from advancing...

...     A new positive selection-based RNAi method Until now, all DNA-based RNAi experiments typically involved...

...secret about science policy that I didn’t learn until arriving here in DC, is that so much policy actually...

...Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and continual development: the power of optogenetics...

...Resources Additional resources on the Addgene blog:  Still trying to choose an antibody? Check out our Selecting...

...have - sometimes you just don’t know who you need until the team really starts functioning (or malfunctioning...

...larger mismatches (e.g. six nucleotides) that may still lead to off-target double-stranded breaks (Tsai ...

...different target. Flow cytometers however are highly versatile and some can accommodate up to 30 different colors...

... overview and comparison of three of the most versatile biosensor biorecognition elements. Biosensors ...

...paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for rapid combinatorial ...

...recombine DNA? There are many methods that have been utilized over the years to move around pieces of DNA. Oftentimes...in the DNA. In restriction cloning, scientists utilize specific restriction enzymes to cut dsDNA of interest...reducing the amount of time required for cloning. To utilize this approach, the fragment of DNA that you would...pairs adenine (A) and thymine (T). TOPO® cloning utilizes the Taq polymerase which naturally leaves a single...traditional restriction enzyme cloning. Scientists have utilized this approach to create compatible custom overhangs...polymerase will continue to function as an exonuclease until a base is exposed on the single strand overhang ... 100kb) compared to other cloning methods that utilize bacteria where it becomes progressively more difficult...

...for light-induced cAMP modulation. Utilizes BLUF (blue light utilizing FAD) domains. 441 Phytochromes BphP1...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...domains from two different ChRs. These ChR variants still function as light-gated, cation channels resulting...used, there is an incubation time (days to weeks) until peak expression of the opsin is reached. This approach...figure above, this light induced binding can be utilized to control the localization of a protein of interest...

...cross the blood-brain barrier in rats, and the utility of DREADDs in experiments in this species may be... as laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent modules...reporter ensures that the reporter remains silent until a cell or tissue-specific Cre is provided. Want ...genetically-encoded bioluminescent opsins for versatile, scalable, and hardware-independent optogenetic...

...transfer plasmid. 2nd generation lentiviral plasmids utilize the viral LTR promoter for gene expression, whereas...whereas 3rd-generation transfer plasmids utilize a hybrid LTR promoter (more information on this below)...basically shuffling restriction sites between vectors until the gene of interest is flanked by sites compatible...

... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...genetic elements. Types of Plasmids Plasmids are versitile and can be used in many different ways by scientists..., like Sanger, is based on DNA replication and utilizes modified fluorescently tagged nucleotides. During...