Hot Plasmids: Summer 2024
Type
Blog Post
...overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of a tardigrade...
We narrowed to 617 results for: TIL;
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Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific... -
10 Steps to a Perfect Science Talk
TypeBlog Post...preparing your talk early - like today Don’t wait until you need to speak to start creating slides or thinking... -
Site Directed Mutagenesis by PCR
TypeBlog Post...University. Site directed mutagenesis is a highly versatile technique that can be used to introduce specific... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...following gates, cells that are not of interest can still be gated out later. Based on personal preference... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...will keep cutting and repairing your target DNA until a mutation is introduced disrupting one or both ... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...negotiations can be much easier. However, negotiations can still go horribly awry when several factors aren’t taken... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...hear too many science trainees get warnings and still think “but that won’t happen to me, I have a backbone... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...Conway AE, Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and... -
A Control for All Seasons
TypeBlog Post... a mouse IgG2a isotype primary antibody but you still observe a signal from your anti-Mouse IgG2a secondary... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...efficiency in pancreatic beta and alpha cells utilizing various AAV serotypes. Scientific Reports, 15(... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...designs, we have observed that certain GRSMs may still not perform as expected, perhaps due to idiosyncrasies... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...and accessibility, more researchers have been utilizing full plasmid sequencing services to verify the... -
Plasmids 101: Repressible Promoters
TypeBlog Post...Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile Expression System for Drosophila. Methods Mol ... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...common problems that many others have faced yet, still, this challenge did not hinder them from advancing... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and continual development: the power of optogenetics... -
Antibodies 101: Validation
TypeBlog Post...Resources Additional resources on the Addgene blog: Still trying to choose an antibody? Check out our Selecting... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post... A new positive selection-based RNAi method Until now, all DNA-based RNAi experiments typically involved... -
Finding Your Science Policy Path
TypeBlog Post...secret about science policy that I didn’t learn until arriving here in DC, is that so much policy actually... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...larger mismatches (e.g. six nucleotides) that may still lead to off-target double-stranded breaks (Tsai ...