We narrowed to 937 results for: TIM

...set of neurons can then be targeted with optical stimuli. The restricted light exposure to a specific area...opsin expression in their cell bodies. Optical stimulation in a different brain area can then reveal the...

...the host plant genome. Scientists have worked to optimize this system for gene transfer, studying the stability...switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they achieved much higher...

...be crucial for a successful application. It takes time to prepare an excellent resume…it is worth it. Read...connections to the hiring group.  Read here for more on timely submission of a job application. 3) Read job descriptions...

...  Dr. Bourne strongly believes this is an amazing time of promise for science—we have powerful tools, beautiful...beautiful questions, and smart scientists.  At the same time, he pointed out, academic research has some big ...

... we can’t localize one cell at a depth of one centimeter with fluorescence is because the light becomes...relevant papers, and then read them again. Most of the time, the answers will be there.     References: Bright...

...vector purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable...place for an hour at 37°C. This should be enough time to completely digest the template DNA. Note—templates...

... purification is still possible but requires optimization and often more stringent washing. Nonetheless...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

... still what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.”...department, and operations department with the ultimate goal of determining if projects are feasible. ...

...Hans uses for his job. “I am really more of a multimedia science communicator than a science writer these...support them. Many thanks to Hans for taking the time to speak with us! Follow Hans on Twitter @HansPacker...

...plants and that produce compounds with various antimicrobial activities (1). She has developed a fast endophyte...Improved Performance for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical Biology 2013 Mar 15;8(3)...

...monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes...Use to track a process (e.g. enzyme activity) over time or under various conditions Localization within...

...fluorophores and luciferases to track bacteria in real time, but each of these reporters has its drawbacks. ...bacteria, Bourdeau et al created two constructs optimized for expression in E. coli: acoustic gene 1 (arg1...

...kanamycin resistance cassette will grow. However, often times, the plasmid backbone includes an antibiotic resistance...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...

...mice to gather the following types of data: Report time-course and concentration of released DA in mouse... dynamics in response to a threatening looming stimulus in vivo in transgenic zebrafish Report DA release...

...antibodies in llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller...name.  We’ve talked about sdAbs a number of times on this blog over the years and have described how...

...cancerous, hybridoma, etc.), as well as sex and age at time of sampling if available. STR profiling for human...who got it from who knows where), now is as good a time as ever to find out the true identity of the line...

...making a custom feature in Snapgene. This saves us time as we are often analyzing deposits with many plasmids... to quickly align multiple sequences at the same time. This means we can easily compare and analyze any...

...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ...for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR...

...to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect...this format and it becomes even more effective over time if you take it seriously.  Person who will receive...

... experiment has not worked…again…for the seventh time….   I find that once I sit down and talk to a scientist...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...