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  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...However, the first two generations of iGluSnFR had limitations: they were not optimal for experiments requiring...photon imaging.   Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V...

  8. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... image, as well as a genetic method for the computational problem of edge detection. Tabor et al. subsequently...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...

  9. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...slower than fluorescent-based methods, such as excitation of Green Fluorescent Protein (GFP) because of...enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression...

  10. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...Potential graduate students typically go through a rotation, which reveals their working habits, personality...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

  12. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... are positive for your marker would be a good statistic to compare between samples. In cases where there...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...

  13. Viral Vectors 101: Biosensors

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    Blog Post
    ...DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte. This signal...biosensor is the signal transducer which will quantitatively give a readout for whatever the bio-recognition...

  14. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...have a major impact on experimental design and interpretation of results. Take extra care when evaluating... present at both the injection site and in the putative projection neurons. When using this serotype to...

  18. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  19. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  20. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...If you missed the webinar, you can watch the presentation and Q&A session on our YouTube channel. And ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...
Showing: 581 - 600 of 836 results