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  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...them that their findings may be the result of a technical artifact that introduced an ancestry bias into... a reduction in the number of the dependencies detected. Given this new information, Misek notes that ...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270-273. PubMed PMID...Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science (2017): eaam9321...

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...Escherichia coli and Agrobacterium tumefaciens. 3 Biotech, 13(3), 91. https://doi.org/10.1007/s13205-023-...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ... a pCAG-EGXXFP plasmid using standard cloning techniques (The XX indicates where your target is inserted...resulting in the expression of EGFP. Using this technique, you can assess several sgRNA’s at the same time...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...Sanger sequencing-based analysis tools are unable to detect or analyze complex edits, such as those generated...sample, ICE includes visual representations of all detected edit types in the sample. The visualization helps...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...terms of the question, the methods, the tools or techniques that can help resolve the question, the anticipated... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...

  11. Lab to Office Culture Shock

    Type
    Blog Post
    ...transferable skills. Transferable skills are the non-technical skills you pick up wherever you work, and you’... communicating. After years of focusing on my technical skills with cloning and lentivirus production,...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...

  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

  14. Bioinformatics at Addgene

    Type
    Blog Post
    ...Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository continues to expand, we realized... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...the presence of liver metastasis by producing a detectable signal in urine. The bottom line with plasmid...Danino, Tal, et al. "Programmable probiotics for detection of cancer in urine." Science translational medicine...

  17. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...microscopy of a viral vector solution. Since this technique is intensive and requires an electron microscope...with titrating by qPCR are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013),...

  18. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...addition to certain staff scientists and those in technical roles. Therefore, institutions are responsible... law. However postdocs, staff scientists and technicians in primarily bench-science positions are not....

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...commonly employed methods for titering IDLVs include detecting vector particle components or performing quantitative...CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt...
Showing: 581 - 600 of 796 results