...luciferase was published in 1985, several studies utilized luciferase as a genetic reporter in plant and ...

...advantage of the many resources available from your institution, your department, and online. The Howard Hughes...

...Your Next Lab" (updated 2020 version) by Addgene Executive Director, Joanne Kamens.   The goal: Finding...

...components greatly reduces the rate of plasmid dilution but does not guarantee 100% transfection efficiency...

...antibodies by applying osmotic pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993)...

... Mix the dilutions well Note: the 1:10 dilution can usually be omitted because this dilution typically... serial dilutions of the lentivirus into DMEM complete containing 10 μg/mL polybrene: Dilution Volume ...quantified. Add 150 μL of a viral dilution to each well (each well gets one dilution) Also include an untransduced... colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate...present in the lower dilutions, and single colonies are visible in the higher dilutions. Last reviewed on...staining resistant colonies with crystal violet solution and, therefore, the cells cannot be used for later...DMEM complete containing 10 μg/mL polybrene by diluting 20 μL of 10 mg/mL polybrene into 20 mL of media...

...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...

...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured ...

... S., Wolf, Y. I., Iranzo, J., et al. (2019). Evolutionary classification of CRISPR–Cas systems: a burst...

...Lentiviruses have gone through their fair share of lab evolution. There are 1st, 2nd, and 3rd generation plasmids...

..., H., Kelly, R.M. and Dijkhuizen, L. Directed evolution of enzymes: Library screening strategies. IUBMB...

...research proposal is the perfect fit to their institutional focus and that you're better equipped than anyone...

...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...

...edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available...

...hybridomas will survive this step. Then, a limiting dilution is performed to isolate individual hybridomas ...

...activated upon binding of CNO thus showing no constitutive activity. There are an array of DREADDs that...

...between organisms. It plays a key role in bacterial evolution and is the primary mechanism by which bacteria...

...the fluorescent protein properties need to be scrutinized under relevant conditions. The properties of ...

...luminescence to get around these issues, but this solution wasn’t practical due to the low intensity of luminescent...

...control signaling provides greater spatiotemporal resolution than pharmacological or genetic manipulation ...