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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 986 results for: des

Showing: 581 - 600 of 986 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...good starting point for most researchers as it provides a list of putative off-target sites that they ... approach misses larger mismatches (e.g. six nucleotides) that may still lead to off-target double-stranded...contains both the rare off target event and the desired edit is low. Therefore, unbiased sequencing may...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...in the development of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary... in different cell types, available at www.crisprbehive.design. CGBEs fused to pioneer factors to improve...strand that does not base pair with the guide RNA. Despite being called the "non-target strand" in CRISPR ...

  5. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    ...quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for...on how Addgene has used Nextflow to improve and redesign our pipeline. In it, they break down the unique...

  6. The Twelve Days of CRISPR

    Type
    Blog Post
    ...#12DaysOfCRIPSR hashtag to share new CRISPR content designed to help you with your CRISPR experiments and to...CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the fourth day of...

  7. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...distribution. Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents in your...materials quickly and easily. Learn more about using barcodes. Check out our “Lab Tip” filter on the blog...

  8. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...anniversary! We've done so much in the past two (2!) decades that it's hard to summarize, but we wanted to mark...experience we're sharing through blogs, eBooks, videos, guides, protocols, and of course, the friendly folks at...

  11. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...10,000 plasmid deposits per year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach...globally. *All data presented in this blog post includes data until November 9, 2020. Between then and ...

  12. CRISPR Cheat Sheet

    Type
    Blog Post
    ...CRISPR 101 eBook. This new edition of the eBook includes the details on advances in CRISPR including: ...bacteria that allow the bacteria to target and destroy viruses that infect them. Commonly referred to ...

  13. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...below the ordering table. Click on it, select your desired serotype and volume, and submit your request.  ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll...

  14. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...of lab materials to create their decorations! Despite all the craziness of 2020, for our Zoom #HolidayParty...microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com...

  15. Editor's Choice, July 2016

    Type
    Blog Post
    ...and point out one or more additional posts that deserve a peek in case you missed them. July 2016 was a...Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET flurophores for a variety...

  16. A Scientist's Guide to Ebola

    Type
    Blog Post
    ... the United States while caring for a patient, despite wearing protective gear, and now comes news of ...Virginia School of Medicine have also recently described how the glycoprotein transforms itself to escape...

  17. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox...IT10 expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system...

  18. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...is absolutely fascinating to see the living art designed by creative minds with a (I assume) very steady...Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology ...
Showing: 581 - 600 of 986 results