Viral Production at Addgene
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Blog Post
...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...
We narrowed to 630 results for: emb
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Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...identified. While LY6A or other Ly6 gene family members are linked to viral susceptibility (mouse adenovirus... -
Hot Plasmids Spring 2024
TypeBlog Post...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post... on the expertise of the senior leadership and members of their consulting staff. Who would enjoy healthcare... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...., Rubinstein, M. “LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis... -
Plasmids 101: Control Plasmids
TypeBlog Post...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central... -
Tips for Using FRET in Your Experiments
TypeBlog Post...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf... -
Transferable Skills Guide: Time Management
TypeBlog Post...physically cross things out (it just feels good) and I remember things better when I write them down. Some people... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...excellent efficiency using a commercial isothermal assembly master mix. Introduction of the strong transcriptional... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...running your analysis. In this case, I and other lab members had worked with lymphocytes before, so I knew where... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...deliver these components to your target cells. Remember, efficient delivery of CRISPR components is essential... -
Evolution of Lab Techniques
TypeBlog Post...smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization of a radioactively... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi:10.1038/nature.2015.18947... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...Attribution License. Within the nervous system, remember that some serotypes can be transported retrogradely... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...this might be frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which cannot distinguish...