We narrowed to 637 results for: erf

1. How to Be an Excellent Trainee

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   Blog Post

   ...enough information there for you to be independent performing the technique you trained to do? Walk through...

2. CRISPR-mediated Plant Base Editors

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   ...G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally known...

3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   ...discovery, plasmid addiction systems have become a powerful tool to address a diverse set of biological inquiries...

4. Plasmids 101: Codon usage bias

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   ... in your expression host of choice, you should perform a functional assay to make sure your protein isn...

5. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

6. Transferable Skills Guide: External Collaborations

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   ...you realize. Being at a university provides the perfect platform for practicing initiative and for training...

7. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   ...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

8. Plasmids 101: Gateway Cloning

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   ... vector.  Once the BP and/or LR reactions are performed, the next step is to transform competent E. coli...

9. Using Video to Share Your Science: We Share Science

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   ... for diagnosing and resolving organizational performance and often have to be able to quickly synthesize...

10. 5 Reasons to Use Reddit for Science Communication

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    ...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

11. Tips for CRISPR Gene Editing in Mice

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    ...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

12. The Challenges of Cell Culture

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    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

13. Getting the Most from Your Lentiviral Transduction

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    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

14. Important Considerations in Optogenetics Behavioral Experiments

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    ...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

15. Plasmids 101: E. coli Strains for Protein Expression

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    ... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...

16. Plasmids 101: The Promoter Region – Let's Go!

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    ...a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   ...

17. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...

18. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ... intestinal crypt, and since then has become a powerful tool for understanding cell proliferation. Inducible...

19. An Integrin Antibody Toolkit from IPI

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    ...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...

20. Tips for Getting a Faculty Position

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    ...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...