We narrowed to 639 results for: erf

1. Plasmids 101: Codon usage bias

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   Blog Post

   ... in your expression host of choice, you should perform a functional assay to make sure your protein isn...

2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

3. Transferable Skills Guide: External Collaborations

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   ...you realize. Being at a university provides the perfect platform for practicing initiative and for training...

4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

5. Fluorescent Proteins 101: Aptamer Fluorophores

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   ...veggies) Though the aptabeacons can be used as powerful biosensors, the bound protein fluorophores and...

6. Plasmids 101: Gateway Cloning

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   ... vector.  Once the BP and/or LR reactions are performed, the next step is to transform competent E. coli...

7. Using Video to Share Your Science: We Share Science

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   ... for diagnosing and resolving organizational performance and often have to be able to quickly synthesize...

8. 5 Reasons to Use Reddit for Science Communication

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   Blog Post

   ...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

9. Tips for CRISPR Gene Editing in Mice

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   ...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

10. The Challenges of Cell Culture

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    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

11. Getting the Most from Your Lentiviral Transduction

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    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

12. Important Considerations in Optogenetics Behavioral Experiments

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    ...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

13. Plasmids 101: E. coli Strains for Protein Expression

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    ... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...

14. Plasmids 101: The Promoter Region – Let's Go!

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    ...a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   ...

15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...

16. Tips for Getting a Faculty Position

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    ...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...

17. CRISPR 101: Homology Directed Repair

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    ...targeted-break technology coupled with HDR is a powerful site-specific knock-in tool that can be harnessed...

18. Transferable Skills Guide: Conflict Resolution

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    ...form of (hopefully constructive) conflict. As you perform the experiments to address a reviewer’s concerns...

19. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...

20. Transferable Skills Guide: Time Management

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    Blog Post

    ...quick and useful tasks will swiftly give you the wonderful feeling of accomplishment while the longer tasks...