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Showing: 581 - 600 of 961 results

  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...antibody. Perform a second series of wash steps. Activate the signaling molecule and perform the assay...sample with the primary (unconjugated) antibody. Perform a series of wash steps to remove any unbound or...

  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...immunogen. A different type of purification can be performed to isolate antibodies only recognizing your protein...steps keep the cost low compared to other antibody formats. This low barrier to entry makes polyclonal antibodies... detect a protein in low abundance or whose conformational state may change. If your protein might become...

  3. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...background papers you could get your hands on, formulated a hypothesis, and planned out your key experiments...so look at epitope target, source, and other information to make sure you’re not trying to choose between...Antibody-based assays are complex and antibodies may perform differently in different samples and experimental...

  4. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...very interesting talk by Prof. Erin M Schuman, a former Caltech researcher, now Director at the Max Planck... from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized...provides both quantitative and spatio-temporal information about the turnover of specific endogenous proteins...

  5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...gene in frame with an MCS. E. coli that were transformed with the empty vectors expressed the ccdB gene...applications. While these two strains ultimately perform the same function, there is some evidence that ...that ccdB Survival™ can be more difficult to transform, depending upon the specific plasmids being used. ...

  6. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this...used to assemble multigene constructs capable of performing production and subsequent necessary modification... them to these organisms via another optimized platform. Gene Stacking As the name implies, gene stacking...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...packaging systems were designed to prevent the formation of replication competent lentivirus (RCL). Replication...requires the viral transactivator, Tat. For more information on the different generations of lentiviral packaging...to obtain the best titer possible. While this information provides a great starting point, it may not translate...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...CeNGEN is planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check ... C. elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative...PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic acids...

  10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...the six months of my co-op, I have conducted informational interviews with nine Addgenies. There are a ...interested to learn more when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me...flexibility and be up to date with the current world. Bioinformatics it is.’” Though she misses the bench, the change...

  11. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice All of these...promoter plasmids! Comparing synthetic promoters performance in mouse, monkey, and human retinas Next, the...in monkey and human retinas. By comparing the performance of promoters across these three species, the ...

  12. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...symposium includes a panel discussion and keynote by former congressman and CEO of the American Association...bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org/advocating-for-science-boston...symposium will culminate with a keynote address by former congressman and CEO of the American Association...

  13. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...understanding the differences can help you make an informed decision. Mammalian cells Mammalian cells are ...Ararat, M., & Graham, F. L. (2002). Preferential transformation of human neuronal cells by human adenoviruses... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

  14. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting’ screens with ...PRIDICT2.0 score. For the best prime editing performance, we suggest that users select some of the best...learning–based model with high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org...

  15. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...consider Gibson cloning. In this technique, you first perform a round of PCR to generate DNA fragments with 15...your insert, DpnI digest away the templates, and transform the PCR products into E. coli. Ligases within ...available cloning kits that one could choose from to perform these experiments. In most cases, there will be...

  16. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...project will not involve proprietary compounds or information. Where publication is expected, the process is...ensure that it does not divulge any propriety information. Working as a team: Industry is all about teamwork...at once. Many companies will hire a postdoc to perform a specific project, while others will hire a group...

  17. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...submitted was hilarious, full of interesting information, and received a lot of attention on social media...Museum and others when I was trying to find local informal science educators, and ever since, the State Museum...the network I built during graduate school is my informal advisory board. Although my goals may not have...

  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents...What are the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries...your phenotype of interest. For more detailed information on CRISPR screens check out Addgene’s 2016 publication...

  19. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... up after trying out the new protocols.io beta platform for sharing, annotating, and storing life science...associated protocols. When you upload your plasmid information, you’ll have the option to add additional documents...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...resulted in 15% C-to-U editing activity. They then performed sixteen rounds of directed evolution across the... cytidine deaminase activity. While RESCUE can perform C-to-U edits, the authors note that it retains ...whole-transcriptome RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A...
Showing: 581 - 600 of 961 results