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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 976 results for: lars;

Showing: 581 - 600 of 976 results

  2. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with UCSF ChimeraX...DNA to read its sequence. Nature Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038...

  3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal...PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth muscle cells from human...

  4. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ... here in New England- the majority of which are solar, wind, or hydroelectric in nature. The operation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

  5. Call for Guest Bloggers

    Type
    Blog Post
    ...expertise through our blog. Do you have skill in a particular technique? Do you have a set of tips you give... An overview of software tools useful to any molecular biologist Tips for protein purification Replica...Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR...

  6. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...fluorescent markers or protein tags, and suitable for molecular cloning. Plus, we decided to include 10 plasmids...µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such as cloning, digestions... Learn about our other beta projects Read our Molecular Biology Reference ...

  7. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... countries. Guides and protocols were our most popular content, while our beloved blog received 1.3 million...scientists in this rapidly changing environment. In particular, we are working to understand what challenges...Addgene colleagues, collaborators, partners, and the larger scientific community to maximize the positive impact...

  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...microscopes work as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron... to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research he applies...applies naturally occurring bacterial “molecular buoyancy devices” (also known as gas vesicles) as acoustic...

  10. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...like GFP came to be used as research tools is particularly interesting. Although humans have known about...crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of ...Soon, the number of people studying it grew. In particular, studies in the laboratory of UCSD biochemist...

  11. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...without controversy, and it remains to be seen how popular any one of them will become. One thing is for sure...was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top requested plasmid from 2016...peptides. PX459 This is the vector backbone. This particular backbone can be used to transfect a variety of...

  12. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific ...

  13. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (Tsang et al., 2016... perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent...

  14. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...correct phages will test. These includes labs with a large number of different phages or with phages targeting...that phage information was very fragmented. A particular phage researcher may only know how to prepare...networking Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers...

  15. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...production of deletions by paired DSBs may be particularly useful. The first is the study of non-coding...and Harvard Medical School. His lab studies the molecular genetics of blood cell development and disease...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ...

  16. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists, blur the...the more friends they have in common. Only the largest connected part of the follower network is shown...physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting that scientists...

  17. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ... Affinity Reagent Working Group of the Human Biomolecular Atlas Program (HuBMAP) antibody metadata standards.... Users can also recommend or not recommend a particular reagent in the given conditions, along with the...building both the community and the database. They particularly encourage people to upload and share their negative...

  18. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...excellent resume. 2) Follow job boards and check them regularly Use your research skills to determine which job... to take next steps and follow up especially in large companies. It is fine to address the cover letter...might be able to help you. If the organization is large, you will need to be more directed and target someone...

  19. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...follow their passion. “There are many ways to have a large positive impact in society. We need much, much more...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ... you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100... plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...good idea of what that volume looks like in your particular plate. For the remainder of the plates, pour ...
Showing: 581 - 600 of 976 results