Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 850 results for: met

Showing: 581 - 600 of 850 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...actually don’t have to worry about and that is something to recognize! Distributing plasmids, viral service...before it was cool or a necessary result of the #MeToo movement. We are carefully attentive to genuine ...

  2. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV....a large intervening deletion. Not only can this method precisely program large genomic deletions of up...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks."Molecular...

  4. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...al, 2017) and cannot be described by a single parameter. Therefore, it is necessary to know how the fluorescence... (iii) attached to a substrate. These in vitro methods allow for a well-controlled environment, but they...

  5. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ... a month or so of meetings, I began to notice something: even though our scientists and engineers were...likely will need to write protocols, SOPs, and methods sections. The next time you write or read one of...

  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...are no explicit instructions on what to do when something fails. While we’re sure our techs could figure...the contents of a tube with the tube itself. If something happens to the worm that requires a change of ...

  7. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

  10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The ...transcription of most of the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective...

  13. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    .... L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to...Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283–310). Elsevier. https:...

  14. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ... elucidate cellular structures. Overexpression methods flood the cell with protein, which can both interfere...with BioRender.com. A key feature of our methodology is the use of a donor plasmid that contains long...

  15. 6 Tips for Grant Writing

    Type
    Blog Post
    ...these findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas... successful applications, I can't always find something that made them better than my unsuccessful ones...

  16. Tips for Technical Support Calls

    Type
    Blog Post
    ...answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it...a very positive experience, also let us know! Sometimes those few “thank you”s are what get a customer...

  18. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...printer, a laser cutter, woodworking tools, and a metalshop. After two summers in Silicon Valley as a student...
Showing: 581 - 600 of 850 results