...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...tended to have similar reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar...et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...electrical activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic...

...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...athletes, as well as the ethical and health policy implications arising from sport-related concussion.   More...

...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...dozens of other published FRET-based sensors. For example, his team is now applying it to improve upon an...monitoring calcium versus zinc should require the simple swapping of binding domains, in practice optimizing...

...agreement, read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

... requires extensive sample processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted...time. Both are helping researchers explore the complexity of translational control in cellular physiology...

...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...have tried to modify it to improve yields. For example, transfecting the producer cell line with an expression...were always normalized to their genomic titers). Examples include the use of exo-AAV1 for transducing sensory...

... you have something to ask or say. It can be as simple as “it was nice meeting you.” Include follow-up...person on LinkedIn I once helped by answering one simple question so I accepted the connection invitation...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...

...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...and we’ll be adding more as the need arises. For example, we’ve teamed up with the Broad Institute to provide...gRNA library in plasmid format, a researcher must amplify the library, use next-generation sequencing to ...

... from otherwise terminally differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and...just 8 years after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

...Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what...what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been discovered...he says, “In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful...

... and interested in science. “Postdoc hires are simpler because of their documented history in science....you might enjoy working closely with others to accomplish your goals. You must understand your own style...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...

...recommended for all systemic capsid applications. For example, AAV-PHP.eB is typically administered at a total... we are calling “strain-specific tropism.” For example, AAV-PHP.B doesn’t lead to enhanced transgene expression...they can display species-specific tropism. For example, AAV9-X1.1 specifically transduces brain endothelial..."Mouse Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-dependent tropism...capsids were deposited by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids...

...transfection step of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging... to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... the components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning...

...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce... (2). Metabolic assays such as Seahorse can be employed to look at oxygen consumption. Drosophila are ...

...that are related to the imaging strategy and the sample. Practical brightness A protein with a high intrinsic... future application. In figure 2 we provide an example of the comparison of the practical brightness of...to this data. The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous...

...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ...donations. That seed money was used to build a completely self-supporting service so that Addgene is not..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...

...cool because they can transmit information, for example, from one of the 180 regions to another or from...the brain to the rest of the body. Therefore, by simply looking at how neurons are physically connected...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled...