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We narrowed to 938 results for: mpl

Showing: 581 - 600 of 938 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...expressed. Once the design and construction steps are complete, it’s time to make virus! The constructed viral...the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... to achieve different biological outcomes. For example, some viruses integrate into the host genome (lentivirus...

  2. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...athletes, as well as the ethical and health policy implications arising from sport-related concussion.   More...

  3. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

  4. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

  5. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...tended to have similar reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar...et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

  7. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...electrical activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic...

  8. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... of origins of replication out there so, for simplicity’s sake, we've ignored those used in eukaryotic...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell...sensitive; others ori's can be "tricked" into amplifying more copies with the addition of chloramphenicol...

  9. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...dozens of other published FRET-based sensors. For example, his team is now applying it to improve upon an...monitoring calcium versus zinc should require the simple swapping of binding domains, in practice optimizing...

  10. The MTA at Addgene

    Type
    Blog Post
    ...agreement, read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

  11. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... from otherwise terminally differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and...just 8 years after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

  12. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... requires extensive sample processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted...time. Both are helping researchers explore the complexity of translational control in cellular physiology...

  13. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...

  14. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...have tried to modify it to improve yields. For example, transfecting the producer cell line with an expression...were always normalized to their genomic titers). Examples include the use of exo-AAV1 for transducing sensory...

  15. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ... you have something to ask or say. It can be as simple as “it was nice meeting you.” Include follow-up...person on LinkedIn I once helped by answering one simple question so I accepted the connection invitation...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

  16. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...and we’ll be adding more as the need arises. For example, we’ve teamed up with the Broad Institute to provide...gRNA library in plasmid format, a researcher must amplify the library, use next-generation sequencing to ...

  17. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what...what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been discovered...he says, “In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful...

  18. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... and interested in science. “Postdoc hires are simpler because of their documented history in science....you might enjoy working closely with others to accomplish your goals. You must understand your own style...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...recommended for all systemic capsid applications. For example, AAV-PHP.eB is typically administered at a total... we are calling “strain-specific tropism.” For example, AAV-PHP.B doesn’t lead to enhanced transgene expression...they can display species-specific tropism. For example, AAV9-X1.1 specifically transduces brain endothelial..."Mouse Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-dependent tropism...capsids were deposited by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids...

  20. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...comprised of 2 plasmids, pPSU1 and pPSU2, that can be amplified affordably in bacteria. They are compatible with...was also independent of another TTAGGG binding complex, shelterin. Swapping the DNA binding domain of ...that can be combined with your gRNA and repair templates of choice though modular cloning steps. The Voytas...that are sure to be enabled by this toolkit. For examples of the toolkit in action, see Cermak T, et al....
Showing: 581 - 600 of 938 results