Addgene

We narrowed to 758 results for: ncl

Showing: 581 - 600 of 758 results

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New and Upcoming Viral Vectors - May 2020

Type

Blog Post

...neurons. We have expanded our AAV collection to include two new ChR2 constructs that allow for targeted...
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Type

Blog Post

...StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like nonstructural proteins 1 to 16, spike...
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

Type

Blog Post

...and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG...
A Practical Approach to Choosing the B(right)est Fluorescent Protein

Type

Blog Post

...application specific parameters into account, including the specs of your microscope (excitation wavelength...
Screening for Successful Genome Editing with Digital PCR

Type

Blog Post

...will focus on assays to detect NHEJ. These assays include a forward and reverse primer to amplify the target...
Hot Plasmids - May 2022

Type

Blog Post

... remains a challenge due to multiple factors, including limitations of number of flourescent proteins ...
Antibodies 101: ChIP

Type

Blog Post

...into DNA expression and protein interactions, including epigenetic modifications. But it's not an approach...
Sweating the Small Stuff: Details in the Lab

Type

Blog Post

... of making an error. Try a variety of things, including (and maybe especially) suggestions you are initially...
Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

...commercial antibodies come with sodium azide already included in the buffer, which will be noted on the product...
Viral Vectors 101: Viral Vector Elements

Type

Blog Post

... transcription of other relevant viral genes (including the ones listed previously). E3/E1B suppresses...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

...been approved in the US for multiple diseases, including hemophilia B and spinal muscular atrophy. ...
Sharing is Caring: But How to Distribute Open Hardware?

Type

Blog Post

...panel shows workflow of open hardware. Conclusion Addgene is a great example of how sharing of ...
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Type

Blog Post

...the slower exonuclease activity to proceed. By including only one type of dNTP in the reaction mix, the...
Antibodies 101: Flow Cytometry Controls

Type

Blog Post

... the antibodies you are using, you may need to include additional controls like FMO or isotype controls...
How Addgene Creates Content

Type

Blog Post

...team, we identified what applications should be included and what basic information someone new to antibodies...
Plasmids 101: Screens vs. Selections

Type

Blog Post

...Things to consider when selecting an assay system include: How specific is it? Is it high throughput? Will...
GPCRs: How Do They Work and How Do We Study Them?

Type

Blog Post

...be activated by an array of different ligands, including hormones, neurotransmitters, photons or odorants...
7 Tips to Secure a STEAM Internship This Summer

Type

Blog Post

...and cover letter revised by your network, which includes your friends, family members, and professional...
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Type

Blog Post

...is most often used, but other selection markers include vermillion (another gene controlling eye color)...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...described in this post in the graphic below - including some simple comparisons. The easy multiplexing...

Showing: 581 - 600 of 758 results

Results per page:

New and Upcoming Viral Vectors - May 2020

Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

A Practical Approach to Choosing the B(right)est Fluorescent Protein

Screening for Successful Genome Editing with Digital PCR

Hot Plasmids - May 2022

Antibodies 101: ChIP

Sweating the Small Stuff: Details in the Lab

Antibodies 101: Buffers, Storage, and Conjugates

Viral Vectors 101: Viral Vector Elements

Viral Vectors 101: Viruses as Biological Tools

Sharing is Caring: But How to Distribute Open Hardware?

Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Antibodies 101: Flow Cytometry Controls

How Addgene Creates Content

Plasmids 101: Screens vs. Selections

GPCRs: How Do They Work and How Do We Study Them?

7 Tips to Secure a STEAM Internship This Summer

Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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