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We narrowed to 932 results for: omp

Showing: 581 - 600 of 932 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters (Zhang et al., 2019). How does...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...

  2. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    .... Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized... genome. Many of these techniques require more complex DNA assemblies and improvements in these methodologies...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

  4. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ... I asked participants to read short abstracts comparing the mortality rates between two controversial ...and biased. As a result, the control abstract comparing two fictional medical issues – chronic and acute...community. I brought along printed copies of studies comparing the filtration efficacy of different materials...

  5. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then... Vice President. Her current responsibilities encompass overall leadership of the Cell Press editorial...

  6. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...sources. While the importance of understanding these complex processes can’t be overstated, we can also learn...degradation under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were...

  7. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...experience using image analysis software. If you use computer programs to predict protein structures, then you... you might know a thing or two about computer modeling. Make sure to focus on these skills. The only exception... into a communications role instead after she completed her MSc in Experimental Medicine. She is the Community...

  8. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...than a day. Diagnostic restriction digests are comprised of 2 separate steps: 1) incubating your DNA with... construct. Consider buffer and temperature compatibility when digesting with more than one enzyme. Consult...will allow you to approximate the DNA mass of comparably intense samples of similar size. Run the gel ...

  9. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...However, one of the many proteins which are not compatible with regular mesophilic systems are the psychrophilic...chaperones protein resulted in higher levels of growth compared to the E. coli strain expressing the empty vector... Medal in the Undergrad category at the iGEM Competition 2019. He has an active interest in scientific...

  11. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ...gamma-retrovirus are so closely related, their required components are very similar. While plasmids containing virus-specific...between the two systems, other, more general, viral components such as a heterologous envelope or a post-transcriptional...

  12. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...nature, extra safety measures, like replication incompetent viral particles, are required. We will be covering...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which... immunodeficiency virus type 1 preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580...

  13. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...service supporters We would like to thank the companies who supported this project with funding. Alnylam...donations. That seed money was used to build a completely self-supporting service so that Addgene is not...distribution, this project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave...

  14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...to start a more formal mentoring program at my company, but there weren't enough senior people willing... such as pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or... and help each other practice meeting people – compete for fastest to set up a coffee date or most cards...

  15. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... light sensitive and have enhanced conductance compared to current channelrhodopsins. Their new channelrhodopsins...neurons do not express as many copies of the ChRs as compared to direct injection. This lower multiplicity of...better suited for systemic delivery with PHP.eB compared to the existing ChR2(H134R).  In the second experiment...

  16. RNA Extraction Without A Kit

    Type
    Blog Post
    ...or tissue collection method, so biotechnology companies have marketed several products that help to further... RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... is the carryover of DNA that can potentially complicate results of a downstream application such as quantitative...

  17. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ... was also between 1013 - 1014 vg/kg, a dosage comparable to those currently used in human gene therapy...fluorescent voltage indicators, however, fall short compared to GFP-based sensors in terms of response and ...precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...bacteriophage shell protein or a bacterial microcompartment protein. The expression of this fusion is ...

  19. To Each HIS Own

    Type
    Blog Post
    ...proteins within a system. This can be pretty complicated given that a single cell has tens of thousands...strategy a single protein can be isolated from a complex mix using affinity chromatography against an amino...then eluted using a high imidazole buffer that outcompetes the interaction between the protein of interest...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it will proceed to ...that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why...Cas7-11 Cas7-11 is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains...
Showing: 581 - 600 of 932 results