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The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...has made it possible to eliminate geographical restrictions in life science research." Claim Your Award ... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...content that scientists will find useful and interesting than that will simply get people ordering plasmids... -
Management for Scientists: Giving Feedback
TypeBlog Post...is possible in your eyes so their confidence is restored. A good manager does not hold grudges. Never... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post... reagents and money. This was the start of my interest in “stingy science”. Practicing thrift and conservation... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...separate resistance cassettes and your gene of interest is cloned into/inactivates or completely removes... -
Choosing a Good Mentor for Scientists
TypeBlog Post...about workplace bullying featured on the always interesting Tenure, She Wrote blog. Unfortunately, academic... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...viral anti-CRISPR genes. These data highlight an interesting "arms race" between phages and prokaryotes; the... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow...E. coli cells by heatshock with the plasmid of interest. See our transformation page for a detailed heatshock... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...use two antibodies - one against your target of interest and a second against the first antibody. When ... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...would be wireless, able to monitor outcomes of interest, and stimulate or manipulate responses through... -
Antibodies 101: Epitope Tags
TypeBlog Post...the lookout for aggregation of your protein of interest! Ready to start epitope tagging? References... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...required three components: a master mix, the DNA of interest, and cell extract. Graphical Abstract... -
The Strength of Story Telling
TypeBlog Post... my job I most enjoy is building and telling interesting stories — whether I’m working with a talented... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog Post...mitophagy. Rosella can also be fused to a protein of interest to track the autophagy of that protein. Find ... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...directed repair (HDR) are echoed in the amount of interest in base editors, which alter genomic DNA without... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post... also need to be transfected into the cells of interest. In contrast, RNA guides must be expressed from... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post...strains you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting... -
Gibson Assembly Protocol
TypeProtocol...extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost any two fragments regardless...and screen for the correct plasmid product by restriction digest . Sequence the important regions of your... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...