We narrowed to 794 results for: tec

...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...Escherichia coli and Agrobacterium tumefaciens. 3 Biotech, 13(3), 91. https://doi.org/10.1007/s13205-023-...

... by directly fusing the cargo to dCas9.  This technique has been exploited to localize factors such as...editors, transposases and prime editors. Nat. Biotechnol. 2020 387 38, 824–844 (2020). Moreno-Mateos, ...

...Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository continues to expand, we realized... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

...mediates targeted genome editing in plants. Plant Biotechnology Journal, 22(10), 2950–2953. https://doi.org/...through de novo induction of meristems. Nature Biotechnology, 38(1), 84–89. https://doi.org/10.1038/s41587...

...transferable skills. Transferable skills are the non-technical skills you pick up wherever you work, and you’... communicating. After years of focusing on my technical skills with cloning and lentivirus production,...

...the presence of liver metastasis by producing a detectable signal in urine. The bottom line with plasmid...Danino, Tal, et al. "Programmable probiotics for detection of cancer in urine." Science translational medicine...

...Sanger sequencing-based analysis tools are unable to detect or analyze complex edits, such as those generated...sample, ICE includes visual representations of all detected edit types in the sample. The visualization helps...

...microscopy of a viral vector solution. Since this technique is intensive and requires an electron microscope...with titrating by qPCR are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013),...

...addition to certain staff scientists and those in technical roles. Therefore, institutions are responsible... law. However postdocs, staff scientists and technicians in primarily bench-science positions are not....

... a pCAG-EGXXFP plasmid using standard cloning techniques (The XX indicates where your target is inserted...resulting in the expression of EGFP. Using this technique, you can assess several sgRNA’s at the same time...

...dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population of dark...Sean, and H. R. Pelham. "Use of peptide tagging to detect proteins expressed from cloned genes: deletion ...

... vectors carrying parts of a larger gene. Nat Biotechnol 20:697–701 . https://doi.org/10.1038/nbt0702-...-splicing adeno-associated viral vectors. Nat Biotechnol 23:1435–1439 . https://doi.org/10.1038/nbt1153...

...commonly employed methods for titering IDLVs include detecting vector particle components or performing quantitative...CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt...

...alleles when gene editing.   Figure 1: Techniques to improve editing efficiency.   But what...non-coding DNA region? To this end we developed a technology called CORRECT, which, by undergoing two rounds...

...#68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols for use of this system...PubMed PMID: 24136966. 8. Tomida, J., et al. "Detection of phosphorylation on large proteins by Western...

...research team. For example, if you’re going to need a tech because you’ll be doing mouse experiments, start... current administrative services, managers, and techs’ financial books.  To find personnel salary ranges...

...administrative responsibilities? Is the work (topic and/or technical aspects) applicable to industry if this path interests...Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look at your data and...

...California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments ...countless other systems, whereas import of CRISPR technology into C. elegans has been a transformative innovation...

...truncated tyrosine hydroxylase (TH) promoter for catecholamine neurons or glial fibrillary acidic protein (...Considerations For Precise Neuronal Targeting Using AAV Technologies Resources on Addgene.org Find ready to use...