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We narrowed to 964 results for: tes

Showing: 581 - 600 of 964 results

  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification... the efficacy of our antibodies through stress testing. An aliquot of our recombinant antibodies is stored...aliquots frozen immediately post-harvest. Stress-tested antibodies that are not equally effective are shipped...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...to ~4-10x linearly! This expansion physically separates molecules while maintaining their relative positions...understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux, et al. 2021; Gambarotto, ...new insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biology...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...detection of the product. As an added benefit, replicates are built into the technology. One well (containing...Have a dedicated bench with a dedicated set of pipettes for ddPCR set-up. Prepare the master mix in a ...low as 1C/s. Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been ...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...dependencies detected. Given this new information, Misek notes that in theory, if the DepMap analysis had an equal...and references References Germline variation contributes to false negatives in CRISPR-based experiments...Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, Stephanie M. Fullerton et al. Getting genetic ...

  5. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...to spend their days microinjecting fluorescent conjugates into cells. Fortunately, over the last 20 years... cognate protein. In some cases this property eliminates the need for removing excess dye from the sample...refinements to these systems—smaller tags, brighter conjugates, higher fluorogenicity, photoactivation etc.—...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ... is received in the lab, although this number fluctuates throughout the year (see graph below). Addgene.... Each plate has numbers and letters to give coordinates, from A1 through H12. Each plate is also barcoded...rack that contains five levels that can hold five plates on each level. This rack is located in one of five...

  8. Lab to Office Culture Shock

    Type
    Blog Post
    ...their best interest to do so, sure, but it also eliminates that feeling of floundering one can have when...case at an office job. I’ve frequently told job candidates over the years that one of the things I enjoy...career options are expanding. As someone who coordinates cross-team projects at Addgene, I find that my...

  9. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...describe how each of these kinds of connectivity facilitates reproducibility. Connectivity to other data sources...available for public access, review, and query. Gates Open Research and Wellcome Open Research both accept...Framework, Data Dryad, and Figshare. Any ELN that facilitates reproducibility has to make it easy to export...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...HDR-mediated gene editing is most efficient when target sites are located in close proximity to the region to ...editing than wild-type SpCas9, even when targeting sites that are known to produce off-target editing with...engineering was Cas12a (formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting...

  11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...-Beier et al., 2019. The team put TREE to the test by infecting HEK-293 cells with the BFP variant,...al., 2020. As a proof of concept the Dow lab tested their hypothesis with GFP fluorescence. To do this...

  12. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...demonstrated that targeting dCas9 to transcriptional start sites was sufficient to repress transcription by blocking...of activators include the SunTag system which facilitates recruitment of multiple activation domains to...2020). Moreno-Mateos, M. A. et al. CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled...

  13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...role of the MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches. What is Rett... the work of the consortium, two gene therapy candidates advanced into clinical development programs in... syndrome gene therapy improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002)...

  14. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...” and AIBS uses it to find potential enhancer candidates. To use these sequences for making functional...the brain. Figure 2: Selection, prep, and testing of AAV enhancers in mice. Adapted from the Allen...collaborative effort that the Allen Institute contributes to. Enhancing science How research is conceived...

  15. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ... the most popular options. Nanopore sequencing deviates from the synthesis-based sequencing methods (Figure...are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence. However, if there...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...grows resulting in the appearance of unmethylated sites in the chromosome. The more stable endonuclease ...an immune response. As a proof of concept, they tested a lux-mediated bioluminescence reporter in vivo...hok/sok system of plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping...

  17. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...increase from $23,660 to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is... must be above this level, and certain job duty tests must be met. In the Department of Labor’s general...postdocs should be paid according to the National Institutes of Health National Research Service Award (NRSA...

  18. Chemogenetics Guide

    Type
    Guide
    ...luciferin) is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite or ...activated through chemogenetic or optogenetic routes. Image courtesy of Ute Hochgeschwender . Table 3. Descriptions...that bind a specific ligand. Ligand binding then activates G-proteins to modulate downstream signaling. GPCRs...back-metabolized to clozapine and other clozapine metabolites and these can have off-target effects. More recent...allows for influx of Na + and/or K + ions, and activates neuronal activity. Figure 3: PSAM4-based ligand-gated...RHJ, DiBerto JF, et al. (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of...

  19. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...saying something like, “I’ll be there in a few minutes, I just need to split some cells.” Inevitably, ... Word or google doc that I can comment and make notes on as I progress. I’ve also recently started using...Business Review article on procrastination recommends testing out how much time you can possibly handle doing...

  20. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...advantage of this system is that streptavidin conjugates are commercially available for a wide range of... heterogeneous cell mixes, like bulk lympho-/splenocytes, you might only be interested in one of the various...mummification). Permeabilization, as the name suggests, perforates the cell membrane to allow for antibodies to ...
Showing: 581 - 600 of 964 results